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Literature summary for 5.1.3.30 extracted from

  • Zhang, W.; Fang, D.; Zhang, T.; Zhou, L.; Jiang, B.; Mu, W.
    Characterization of a metal-dependent D-psicose 3-epimerase from a novel strain, Desmospora sp. 8437 (2013), J. Agric. Food Chem., 61, 11468-11476.
    View publication on PubMed
Search for more literature for 5.1.3.30

Application

Application Comment Organism
synthesis synthesis of the rare sugar D-psicose, that is an ideal sucrose substitute for food products, due to having 70% of the relative sweetness but 0.3% of the energy of sucrose. It also shows important physiological functions Desmospora sp. 8437

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Desmospora sp. 8437

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
81.3
-
 D-psicose pH 7.5, 60°C Desmospora sp. 8437
549
-
 D-fructose pH 7.5, 60°C Desmospora sp. 8437

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ the enzyme is strictly metal-dependent and displays maximum activity in the presence of Co2+ Desmospora sp. 8437
Fe2+ the enzyme is strictly metal-dependent and displays maximum activity in the presence of Co2+. When Co2+ is replaced with Fe2+ the enzyme activity is reduced to 49% of that in the presence of Co2+ Desmospora sp. 8437
Mn2+ the enzyme is strictly metal-dependent and displays maximum activity in the presence of Co2+. When Co2+ is replaced with Mn2+ the enzyme activity is reduced to 80% of that in the presence of Co2+ Desmospora sp. 8437
additional information Mg2+, Zn2+, and Cu2+ can not excite activity Desmospora sp. 8437
Ni2+ the enzyme is strictly metal-dependent and displays maximum activity in the presence of Co2+. When Co2+ is replaced with Ni2+, the enzyme activity is reduced to 14% of that in the presence of Co2+ Desmospora sp. 8437

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 4 * 33000, SDS-PAGE Desmospora sp. 8437
132000
-
 gel filtration Desmospora sp. 8437

Organism

Organism UniProt Comment Textmining
Desmospora sp. 8437
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Desmospora sp. 8437

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose the equilibrium ratio between D-psicose and D-fructose is 30:70 Desmospora sp. 8437 D-psicose
-
 r
D-psicose the equilibrium ratio between D-psicose and D-fructose is 30:70 Desmospora sp. 8437 D-fructose
-
 r

Subunits

Subunits Comment Organism
tetramer 4 * 33000, SDS-PAGE Desmospora sp. 8437

Synonyms

Synonyms Comment Organism
DTEase ambiguous Desmospora sp. 8437

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Desmospora sp. 8437

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 70 50°C: about 50% of maximal activity, 70°C: about 55% of maximal activity Desmospora sp. 8437

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 2 h, 46% loss of activity Desmospora sp. 8437
50
-
 2 h, 42% loss of activity, thermostability property is almost not affected by the addition of Co2+ Desmospora sp. 8437
60
-
 the enzyme loses all of the initial activity after 0.5 h of exposure, thermostability property is almost not affected by the addition of Co2+ Desmospora sp. 8437

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
85.95
-
 D-psicose pH 7.5, 60°C Desmospora sp. 8437
63570
-
 D-fructose pH 7.5, 60°C Desmospora sp. 8437

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Desmospora sp. 8437

pH Range

pH Minimum pH Maximum Comment Organism
6 9 pH 6.0: about 70% of maximal activity, pH 9.0: 75% of maximal activity Desmospora sp. 8437