Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Ruminiclostridium cellulolyticum |
General Stability | Organism |
---|---|
the enzyme is extremely thermostable in the presence of Co2+ but readily inactivated without metal ion | Ruminiclostridium cellulolyticum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
17.4 | - |
D-psicose | pH 8.0, 55°C | Ruminiclostridium cellulolyticum | |
53.5 | - |
D-fructose | pH 8.0, 55°C | Ruminiclostridium cellulolyticum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | the enzyme is strictly metal-dependent and shows a maximal activity in the presence of Co2+ | Ruminiclostridium cellulolyticum | |
Fe2+ | the enzyme is strictly metal-dependent and shows a maximal activity in the presence of Co2+. When Co2+ is replaced with Fe2+ the enzyme activity is reduced to 67% of that in presence of Co2+ | Ruminiclostridium cellulolyticum | |
Mg2+ | the enzyme is strictly metal-dependent and shows a maximal activity in the presence of Co2+. When Co2+ is replaced with Mg2+ the enzyme activity is reduced to 38% of that in presence of Co2+ | Ruminiclostridium cellulolyticum | |
Mn2+ | the enzyme is strictly metal-dependent and shows a maximal activity in the presence of Co2+. When Co2+ is replaced with Mn2+ the enzyme activity is reduced to 92% of that in presence of Co2+ | Ruminiclostridium cellulolyticum | |
Ni2+ | the enzyme is strictly metal-dependent and shows a maximal activity in the presence of Co2+. When Co2+ is replaced with Ni2+ the enzyme activity is reduced to 65% of that in presence of Co2+ | Ruminiclostridium cellulolyticum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
4 * 32000, SDS-PAGE | Ruminiclostridium cellulolyticum |
130000 | - |
gel filtration | Ruminiclostridium cellulolyticum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ruminiclostridium cellulolyticum | B8I944 | - |
- |
Ruminiclostridium cellulolyticum ATCC 35319 | B8I944 | - |
- |
Purification (Comment) | Organism |
---|---|
nickel-affinity chromatography | Ruminiclostridium cellulolyticum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose | the enzyme carries out the epimerization of D-fructose to D-psicose with a conversion yield of 32% under optimal conditions | Ruminiclostridium cellulolyticum | D-psicose | - |
r | |
D-fructose | the enzyme carries out the epimerization of D-fructose to D-psicose with a conversion yield of 32% under optimal conditions | Ruminiclostridium cellulolyticum ATCC 35319 | D-psicose | - |
r | |
D-psicose | the enzyme carries out the epimerization of D-fructose to D-psicose with a conversion yield of 32% under optimal conditions | Ruminiclostridium cellulolyticum | D-fructose | - |
r | |
D-psicose | the enzyme carries out the epimerization of D-fructose to D-psicose with a conversion yield of 32% under optimal conditions | Ruminiclostridium cellulolyticum ATCC 35319 | D-fructose | - |
r | |
additional information | the relative activity with D-tagatose is 4.9% of the activity with D-psicose, the catalytic efficiency for D-tagatose is 249fold lower than the catalytic efficiency for D-fructose, the relative activity with D-sorbose is 0.9% of the activity wth D-psicose. No activity with D-fructose-6-phosphate or D-ribulose-5-phosphate | Ruminiclostridium cellulolyticum | ? | - |
? | |
additional information | the relative activity with D-tagatose is 4.9% of the activity with D-psicose, the catalytic efficiency for D-tagatose is 249fold lower than the catalytic efficiency for D-fructose, the relative activity with D-sorbose is 0.9% of the activity wth D-psicose. No activity with D-fructose-6-phosphate or D-ribulose-5-phosphate | Ruminiclostridium cellulolyticum ATCC 35319 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 32000, SDS-PAGE | Ruminiclostridium cellulolyticum |
Synonyms | Comment | Organism |
---|---|---|
ACL75304 protein | - |
Ruminiclostridium cellulolyticum |
Ccel_0941 | - |
Ruminiclostridium cellulolyticum |
DPEase | ambiguous | Ruminiclostridium cellulolyticum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Ruminiclostridium cellulolyticum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 70 | 35°C: about 50% of maximal activity, 70°C: about 65% of maximal activity | Ruminiclostridium cellulolyticum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
the half-lives for the enzyme are 9.5 h and 24 min when incubated with and without Co2+ (0.1 mM) | Ruminiclostridium cellulolyticum |
60 | - |
the half-lives for the enzyme are 6.8 h and 10 min when incubated with and without Co2+ (0.1 mM) | Ruminiclostridium cellulolyticum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
54.1 | - |
D-psicose | pH 8.0, 55°C | Ruminiclostridium cellulolyticum | |
55.9 | - |
D-fructose | pH 8.0, 55°C | Ruminiclostridium cellulolyticum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Ruminiclostridium cellulolyticum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | 6.0: about 60% of maximal activity, pH 9.0: about 70% of maximal activity | Ruminiclostridium cellulolyticum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | - |
4°C, 2 h, 15% loss of activity | Ruminiclostridium cellulolyticum |
6.5 | - |
4°C, 2 h, 10% loss of activity | Ruminiclostridium cellulolyticum |
7 | - |
4°C, 2 h, 5% loss of activity | Ruminiclostridium cellulolyticum |
7.5 | 8 | 4°C, 2 h, stable | Ruminiclostridium cellulolyticum |
8.5 | 9 | 4°C, 2 h, 10% loss of activity | Ruminiclostridium cellulolyticum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
D-fructose | pH 8.0, 55°C | Ruminiclostridium cellulolyticum | |
3.1 | - |
D-psicose | pH 8.0, 55°C | Ruminiclostridium cellulolyticum |