Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Clostridium sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
227.6 | - |
D-psicose | pH 8.0, 65°C, in presence of 0.2 mM Co2+ | Clostridium sp. | |
279 | - |
D-fructose | pH 8.0, 65°C, in presence of 0.2 mM Co2+ | Clostridium sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | the metal-dependent enzyme requires Co2+ as optimum cofactor. The relative activity is linearly increased when adding Co2+ from 0.005 mM to 0.035 mM. When adding greater concentrations of Co2+, the relative activity tends to be stable. The optimum Co2+ concentration is 0.2 mM for catalytic activity | Clostridium sp. | |
Mg2+ | the metal-dependent enzyme requires Co2+ as optimum cofactor. When Co2+ is replaced with Mg2+ the enzyme activity is reduced to 22% of that in presence of Co2+ | Clostridium sp. | |
Mn2+ | the metal-dependent enzyme requires Co2+ as optimum cofactor. When Co2+ is replaced with Mn2+ the enzyme activity is reduced to 66% of that in presence of Co2+ | Clostridium sp. | |
Ni2+ | the metal-dependent enzyme requires Co2+ as optimum cofactor. When Co2+ is replaced with Ni2+ the enzyme activity is reduced to 31% of that in presence of Co2+ | Clostridium sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium sp. | H2JEN2 | - |
- |
Clostridium sp. BNL1100 | H2JEN2 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-affinity chromatography | Clostridium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-psicose | optimum substrate. Equilibrium ratio between D-psicose and D-fructose is 28:72 at 65°C | Clostridium sp. | D-fructose | - |
r | |
D-psicose | optimum substrate. Equilibrium ratio between D-psicose and D-fructose is 28:72 at 65°C | Clostridium sp. BNL1100 | D-fructose | - |
r |
Synonyms | Comment | Organism |
---|---|---|
Clo1100_1157 | locus name | Clostridium sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Clostridium sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 75 | 50°C: about 50% of maximal activity, 75°C: about 70% of maximal activity | Clostridium sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
4 h, stable | Clostridium sp. |
50 | - |
4 h, about 50% loss of activity | Clostridium sp. |
60 | - |
half-life without metal: 15 min | Clostridium sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
273 | - |
D-fructose | pH 8.0, 65°C, in presence of 0.2 mM Co2+ | Clostridium sp. | |
536 | - |
D-psicose | pH 8.0, 65°C, in presence of 0.2 mM Co2+ | Clostridium sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Clostridium sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | pH 6.0: about 80% of maximal activity, pH 9.0: about 90% of maximal activity | Clostridium sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | 4°C, 2 h, more than 80% of initial activity remains | Clostridium sp. |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.98 | - |
D-fructose | pH 8.0, 65°C, in presence of 0.2 mM Co2+ | Clostridium sp. | |
2.4 | - |
D-psicose | pH 8.0, 65°C, in presence of 0.2 mM Co2+ | Clostridium sp. |