Application | Comment | Organism |
---|---|---|
synthesis | suitable for the industrial production of D-psicose from fructose | Ruminococcus sp. |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Ruminococcus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | leads to protein aggregation and thus drastically decreased activity | Ruminococcus sp. | |
Ni2+ | leads to protein aggregation and thus drastically decreased activity | Ruminococcus sp. | |
Zn2+ | leads to protein aggregation and thus drastically decreased activity | Ruminococcus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
48 | - |
D-psicose | pH 8.0, 60°C | Ruminococcus sp. | |
216 | - |
D-fructose | pH 8.0, 60°C | Ruminococcus sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe3+ | at 1 mM increases enzymatic activity by 30% | Ruminococcus sp. | |
Mg2+ | at 1 mM increases enzymatic activity by 10% | Ruminococcus sp. | |
Mn2+ | at 1 mM increases enzymatic activity by 60% | Ruminococcus sp. | |
additional information | activity is not dependent on the presence of metal ions | Ruminococcus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
3 * 33000, SDS-PAGE | Ruminococcus sp. |
33356 | - |
4 * 33356, calculated from sequence | Ruminococcus sp. |
132000 | - |
gel filtration | Ruminococcus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ruminococcus sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Ruminococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose | the equilibrium ratio between D-psicose and D-fructose is 28:72 at 60°C | Ruminococcus sp. | D-psicose | - |
r | |
D-psicose | the equilibrium ratio between D-psicose and D-fructose is 28:72 at 60°C | Ruminococcus sp. | D-fructose | - |
r | |
additional information | catalytic efficiency with D-tagatose is 108fold lower than cataltic efficiency with D-psicose | Ruminococcus sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 3 * 33000, SDS-PAGE | Ruminococcus sp. |
tetramer | 4 * 33356, calculated from sequence | Ruminococcus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Ruminococcus sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 70 | 40°C: about 60% of maximal activity, 70°C: about 60% of maximal activity | Ruminococcus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
the thermostability of the enzyme is enhanced by the addition of Mn2+ | Ruminococcus sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
40.45 | - |
D-psicose | pH 8.0, 60°C | Ruminococcus sp. | |
59.4 | - |
D-fructose | pH 8.0, 60°C | Ruminococcus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | - |
Ruminococcus sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | pH 6.0: about 50% of maximal activity, pH 9.0: about 80% of maximal activity | Ruminococcus sp. |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.275 | - |
D-fructose | pH 8.0, 60°C | Ruminococcus sp. | |
0.84 | - |
D-psicose | pH 8.0, 60°C | Ruminococcus sp. |