Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
2 * 39000 | Escherichia coli |
Organic Solvent | Comment | Organism |
---|---|---|
urea | denaturation by 8 M urea at pH 7.0 causes 85% loss of ist secondary structure and dissociation of its constituent molecules | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Renatured (Comment) | Organism |
---|---|
dilution of the denaturant urea by buffer at pH 8.5 leads to functional reconstitution of the dimeric holoenzyme. The refolding process is biphasic: after 2 min an equilibrium conformer is formed having 72% of its native secondary structure and by 60 min reactivation becomes complete. The early intermediate has lower energy of activation against thermal denaturation than the reactivated state | Escherichia coli |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 39000 | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | covalently bound | Escherichia coli |