Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.1.3.17 extracted from

  • Li, K.; Bethea, H.N.; Liu, J.
    Using engineered 2-O-sulfotransferase to determine the activity of heparan sulfate C5-epimerase and its mutants (2010), J. Biol. Chem., 285, 11106-11113.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.1.3.17

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes in Escherichia coli strain DE3 Homo sapiens

Protein Variants

Protein Variants Comment Organism
Y146A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Homo sapiens
Y162A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Homo sapiens
Y168A site-directed mutagenesis, inactive mutant Homo sapiens
Y210A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Homo sapiens
Y222A site-directed mutagenesis, inactive mutant Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane transmembrane enzyme Homo sapiens 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Heparosan-N-sulfate D-glucuronate Homo sapiens
-
 Heparosan-N-sulfate L-iduronate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant enzymes from Escherichia coli strain DE3 Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
heparosan-N-sulfate D-glucuronate = heparosan-N-sulfate L-iduronate proposed catalytic mechanisms of C5-epi, overview Homo sapiens
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Heparosan-N-sulfate D-glucuronate
-
 Homo sapiens Heparosan-N-sulfate L-iduronate
-
 ?
Heparosan-N-sulfate D-glucuronate development of a coupled assay method using the enzyme and a 2-O-sulfotransferase mutant 2OST Y94I, overview Homo sapiens Heparosan-N-sulfate L-iduronate
-
 ?

Subunits

Subunits Comment Organism
More the human epimerase is a 617-amino acid protein that consists of three domains, cytoplasmic comprising residues Met1-Lys11, transmembrane with Thr12-Trp28, and catalytic domain formed by Asn29-Asn617 Homo sapiens

Synonyms

Synonyms Comment Organism
Heparan sulfate C5-epimerase
-
 Homo sapiens
HS C5-epimerase
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Homo sapiens