Cloned (Comment) | Organism |
---|---|
expression in Sf9 insect cells, wild-type enzyme and mutant enzymes DELTA1-39, DELTA1-234, DELTA1-356, DELTA383-722, DELTA490-722, DELTA597-722, DELTA697-722, DELTA717-722 | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
DELTA1-234 | mutant enzyme shows no N-epimerase activity | Rattus norvegicus |
DELTA1-356 | mutant enzyme shows no N-epimerase activity | Rattus norvegicus |
DELTA1-39 | mutant enzyme shows no N-epimerase activity | Rattus norvegicus |
DELTA383-722 | epimerase activity is 2% of wild-type enzyme | Rattus norvegicus |
DELTA490-722 | epimerase activity is 15% of wild-type enzyme | Rattus norvegicus |
DELTA597-722 | epimerase activity is 2% of wild-type enzyme | Rattus norvegicus |
DELTA697-722 | epimerase activity is about 70% of wild-type enzyme | Rattus norvegicus |
DELTA717-722 | epimerase activity is about 95% of wild-type enzyme | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
85000 | - |
6 * 85000, SDS-PAGE | Rattus norvegicus |
500000 | - |
gel filtration | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 85000, SDS-PAGE | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase | bifunctional enzyme | Rattus norvegicus |