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Literature summary for 5.1.3.14 extracted from

  • Chou, W.K.; Hinderlich, S.; Reutter, W.; Tanner, M.E.
    Sialic acid biosynthesis: stereochemistry and mechanism of the reaction catalyzed by the mammalian UDP-N-acetylglucosamine 2-epimerase (2003), J. Am. Chem. Soc., 125, 2455-2461.
    View publication on PubMed

General Stability

General Stability Organism
the purified enzyme is 80% pure, no further purification because it is unstable to freezing and has limited stability upon storage at 4°C Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.026
-
UDP-N-acetyl-D-glucosamine 37°C, pH 7.5 Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Rattus norvegicus the bifunctional enzyme UDP-N-acetylglucosamine 2-epimerase/ManNAc kinase catalyzes the first two steps in the biosynthesis of the sialic acids ?
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme expressed in Spodoptera frugiperda cells using a baculovirus expression system Rattus norvegicus

Storage Stability

Storage Stability Organism
4°C, purified enzyme is 80% pure, no further purification because it is unstable to freezing and has limited stability upon storage at 4°C Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information reaction mechanism involving an anti-elimination of UDP to give 2-acetamidoglucal, followed by a syn-addition of water Rattus norvegicus ?
-
?
additional information the bifunctional enzyme UDP-N-acetylglucosamine 2-epimerase/ManNAc kinase catalyzes the first two steps in the biosynthesis of the sialic acids Rattus norvegicus ?
-
?

Synonyms

Synonyms Comment Organism
More bifunctional enzyme UDP-N-acetylglucosamine 2-epimerase/ManNAc kinase Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.33
-
UDP-N-acetyl-D-glucosamine 37°C, pH 7.5 Rattus norvegicus