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Literature summary for 5.1.3.14 extracted from
- Purification and properties of UDP-N-acetylglucosamine 2'-epimerase from rat liver (1973), Biochim. Biophys. Acta, 327, 193-206.
General Stability
| General Stability | Organism |
|---|---|
| DTT protects against inactivation at 4 C | Rattus norvegicus |
| UDP and UDP-N-acetylglucosamine protect the enzyme from inactivation by aging | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CMP-N-acetylneuraminic acid | 50% inhibition by 0.025 mM | Rattus norvegicus |  |
| UDP | competitive | Rattus norvegicus | |
| UDPglucose | - |
Rattus norvegicus | |
| UMP | - |
Rattus norvegicus | |
| UTP | - |
Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.08 | - |
UDP-N-acetyl-D-glucosamine | and a second Km-value of 0.29 mM | Rattus norvegicus | |
| 0.29 | - |
UDP-N-acetyl-D-glucosamine | and a second Km-value of 0.08 mM | Rattus norvegicus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, in presence of 0.1 mM UDP and 1 mM DTT, 3 days, 20% loss of activity | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-N-acetyl-D-glucosamine | - |
Rattus norvegicus | UDP-N-acetyl-D-mannosamine | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 8.5 | - |
Rattus norvegicus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.025 | - |
CMP-N-acetylneuraminic acid | - |
Rattus norvegicus | |
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