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Literature summary for 5.1.3.11 extracted from
- Immobilization of a thermostable cellobiose 2-epimerase from Rhodothermus marinus JCM9785 and continuous production of epilactose (2012), Biosci. Biotechnol. Biochem., 76, 1584-1587.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene RmCE, recombinant expression in Escherichia coli | Rhodothermus marinus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | immobilization of the purified recombinant enzyme on an anion exchange resin and conversion of lactose to epilactose in a chromatographic column, method development and evaluation, overview | Rhodothermus marinus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lactose | Rhodothermus marinus | - |
epilactose | - |
r |  |
| lactose | Rhodothermus marinus JCM 9785 | - |
epilactose | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodothermus marinus | F8WRK9 | gene RmCE | - |
| Rhodothermus marinus JCM 9785 | F8WRK9 | gene RmCE | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli | Rhodothermus marinus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lactose | - |
Rhodothermus marinus | epilactose | - |
r | |
| lactose | enzyme reaction assay with immobilized enzyme | Rhodothermus marinus | epilactose | - |
r | |
| lactose | - |
Rhodothermus marinus JCM 9785 | epilactose | - |
r | |
| lactose | enzyme reaction assay with immobilized enzyme | Rhodothermus marinus JCM 9785 | epilactose | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cellobiose 2-epimerase | - |
Rhodothermus marinus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
- |
Rhodothermus marinus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 100 | activity range, profile, overview | Rhodothermus marinus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.3 | - |
- |
Rhodothermus marinus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 9 | activity range, profile, overview | Rhodothermus marinus |
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