Cloned (Comment) | Organism |
---|---|
gene RmCE, DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli | Rhodothermus marinus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
27.2 | - |
cellobiose | pH 6.3, 60°C, recombinnat enzyme | Rhodothermus marinus | |
28.8 | - |
lactose | pH 6.3, 60°C, recombinnat enzyme | Rhodothermus marinus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellobiose | Rhodothermus marinus | - |
D-glucosyl-D-mannose | - |
r | |
cellobiose | Rhodothermus marinus JCM 9785 | - |
D-glucosyl-D-mannose | - |
r | |
additional information | Rhodothermus marinus | cellobiose 2-epimerase reversibly converts alpha-glucose residue to alpha-mannose residue at the reducing end of beta-1,4-linked oligosaccharides | ? | - |
? | |
additional information | Rhodothermus marinus JCM 9785 | cellobiose 2-epimerase reversibly converts alpha-glucose residue to alpha-mannose residue at the reducing end of beta-1,4-linked oligosaccharides | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodothermus marinus | F8WRK9 | gene RmCE | - |
Rhodothermus marinus JCM 9785 | F8WRK9 | gene RmCE | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli by ammonium sulfate fractionation, ultrafiltration, and gel filtration | Rhodothermus marinus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-O-beta-D-glucosyl-D-mannose | - |
Rhodothermus marinus | 4-O-beta-D-galactosyl-D-mannose | - |
r | |
4-O-beta-D-glucosyl-D-mannose | - |
Rhodothermus marinus JCM 9785 | 4-O-beta-D-galactosyl-D-mannose | - |
r | |
cellobiose | - |
Rhodothermus marinus | D-glucosyl-D-mannose | - |
r | |
cellobiose | - |
Rhodothermus marinus JCM 9785 | D-glucosyl-D-mannose | - |
r | |
lactose | - |
Rhodothermus marinus | epilactose | - |
r | |
lactose | - |
Rhodothermus marinus JCM 9785 | epilactose | - |
r | |
additional information | cellobiose 2-epimerase reversibly converts alpha-glucose residue to alpha-mannose residue at the reducing end of beta-1,4-linked oligosaccharides | Rhodothermus marinus | ? | - |
? | |
additional information | substrate specificity, overview. No activity with glucose, galactose, mannose, sophorose, laminaribiose, gentiobiose, maltose. Cellobiose 2-epimerase efficiently produces epilactose carrying prebiotic properties from lactose. The enzyme shows preference for lactose compared to cellobiose | Rhodothermus marinus | ? | - |
? | |
additional information | cellobiose 2-epimerase reversibly converts alpha-glucose residue to alpha-mannose residue at the reducing end of beta-1,4-linked oligosaccharides | Rhodothermus marinus JCM 9785 | ? | - |
? | |
additional information | substrate specificity, overview. No activity with glucose, galactose, mannose, sophorose, laminaribiose, gentiobiose, maltose. Cellobiose 2-epimerase efficiently produces epilactose carrying prebiotic properties from lactose. The enzyme shows preference for lactose compared to cellobiose | Rhodothermus marinus JCM 9785 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cellobiose 2-epimerase | - |
Rhodothermus marinus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
- |
Rhodothermus marinus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 90 | high activity within this range | Rhodothermus marinus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
purified recombinant enzyme, stable up to | Rhodothermus marinus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
80.8 | - |
cellobiose | pH 6.3, 60°C, recombinnat enzyme | Rhodothermus marinus | |
111 | - |
lactose | pH 6.3, 60°C, recombinnat enzyme | Rhodothermus marinus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.3 | - |
- |
Rhodothermus marinus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3.2 | 10.8 | purified recombinant enzyme, stable at | Rhodothermus marinus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Rhodothermus marinus | sequence calculation | - |
6.3 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.97 | - |
cellobiose | pH 6.3, 60°C, recombinnat enzyme | Rhodothermus marinus | |
3.85 | - |
lactose | pH 6.3, 60°C, recombinnat enzyme | Rhodothermus marinus |