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Literature summary for 5.1.3.11 extracted from

  • Ojima, T.; Saburi, W.; Sato, H.; Yamamoto, T.; Mori, H.; Matsui, H.
    Biochemical characterization of a thermophilic cellobiose 2-epimerase from a thermohalophilic bacterium, Rhodothermus marinus JCM9785 (2011), Biosci. Biotechnol. Biochem., 75, 2162-2168.
    View publication on PubMed
Search for more literature for 5.1.3.11

Cloned(Commentary)

Cloned (Comment) Organism
gene RmCE, DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli Rhodothermus marinus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
27.2
-
 cellobiose pH 6.3, 60°C, recombinnat enzyme Rhodothermus marinus
28.8
-
 lactose pH 6.3, 60°C, recombinnat enzyme Rhodothermus marinus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cellobiose Rhodothermus marinus
-
 D-glucosyl-D-mannose
-
 r
cellobiose Rhodothermus marinus JCM 9785
-
 D-glucosyl-D-mannose
-
 r
additional information Rhodothermus marinus cellobiose 2-epimerase reversibly converts alpha-glucose residue to alpha-mannose residue at the reducing end of beta-1,4-linked oligosaccharides ?
-
 ?
additional information Rhodothermus marinus JCM 9785 cellobiose 2-epimerase reversibly converts alpha-glucose residue to alpha-mannose residue at the reducing end of beta-1,4-linked oligosaccharides ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rhodothermus marinus F8WRK9 gene RmCE
-
Rhodothermus marinus JCM 9785 F8WRK9 gene RmCE
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli by ammonium sulfate fractionation, ultrafiltration, and gel filtration Rhodothermus marinus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-O-beta-D-glucosyl-D-mannose
-
 Rhodothermus marinus 4-O-beta-D-galactosyl-D-mannose
-
 r
4-O-beta-D-glucosyl-D-mannose
-
 Rhodothermus marinus JCM 9785 4-O-beta-D-galactosyl-D-mannose
-
 r
cellobiose
-
 Rhodothermus marinus D-glucosyl-D-mannose
-
 r
cellobiose
-
 Rhodothermus marinus JCM 9785 D-glucosyl-D-mannose
-
 r
lactose
-
 Rhodothermus marinus epilactose
-
 r
lactose
-
 Rhodothermus marinus JCM 9785 epilactose
-
 r
additional information cellobiose 2-epimerase reversibly converts alpha-glucose residue to alpha-mannose residue at the reducing end of beta-1,4-linked oligosaccharides Rhodothermus marinus ?
-
 ?
additional information substrate specificity, overview. No activity with glucose, galactose, mannose, sophorose, laminaribiose, gentiobiose, maltose. Cellobiose 2-epimerase efficiently produces epilactose carrying prebiotic properties from lactose. The enzyme shows preference for lactose compared to cellobiose Rhodothermus marinus ?
-
 ?
additional information cellobiose 2-epimerase reversibly converts alpha-glucose residue to alpha-mannose residue at the reducing end of beta-1,4-linked oligosaccharides Rhodothermus marinus JCM 9785 ?
-
 ?
additional information substrate specificity, overview. No activity with glucose, galactose, mannose, sophorose, laminaribiose, gentiobiose, maltose. Cellobiose 2-epimerase efficiently produces epilactose carrying prebiotic properties from lactose. The enzyme shows preference for lactose compared to cellobiose Rhodothermus marinus JCM 9785 ?
-
 ?

Synonyms

Synonyms Comment Organism
cellobiose 2-epimerase
-
 Rhodothermus marinus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
 Rhodothermus marinus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 90 high activity within this range Rhodothermus marinus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 purified recombinant enzyme, stable up to Rhodothermus marinus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
80.8
-
 cellobiose pH 6.3, 60°C, recombinnat enzyme Rhodothermus marinus
111
-
 lactose pH 6.3, 60°C, recombinnat enzyme Rhodothermus marinus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3
-
-
 Rhodothermus marinus

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.2 10.8 purified recombinant enzyme, stable at Rhodothermus marinus

pI Value

Organism Comment pI Value Maximum pI Value
Rhodothermus marinus sequence calculation
-
 6.3

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.97
-
 cellobiose pH 6.3, 60°C, recombinnat enzyme Rhodothermus marinus
3.85
-
 lactose pH 6.3, 60°C, recombinnat enzyme Rhodothermus marinus