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Literature summary for 5.1.3.11 extracted from

  • Park, C.S.; Kim, J.E.; Choi, J.G.; Oh, D.K.
    Characterization of a recombinant cellobiose 2-epimerase from Caldicellulosiruptor saccharolyticus and its application in the production of mannose from glucose (2011), Appl. Microbiol. Biotechnol., 92, 1187-1196.
    View publication on PubMed
Search for more literature for 5.1.3.11

Activating Compound

Activating Compound Comment Organism Structure
additional information the enzyme is not affected by ATP Caldicellulosiruptor saccharolyticus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli ER2566 cells Caldicellulosiruptor saccharolyticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
51.8
-
 D-mannose in 50 mM PIPES buffer (pH 7.5), at 75°C Caldicellulosiruptor saccharolyticus
70.9
-
 D-glucose in 50 mM PIPES buffer (pH 7.5), at 75°C Caldicellulosiruptor saccharolyticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
 gel filtration Caldicellulosiruptor saccharolyticus
47340
-
 1 * 47340, calculated from amino acid sequence Caldicellulosiruptor saccharolyticus

Organism

Organism UniProt Comment Textmining
Caldicellulosiruptor saccharolyticus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His-Trap HP column chromatography, gel filtration Caldicellulosiruptor saccharolyticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0095
-
 in 50 mM PIPES buffer (pH 7.5) containing 10 mM D-fructose as substrate at 75°C Caldicellulosiruptor saccharolyticus
0.035
-
 in 50 mM PIPES buffer (pH 7.5) containing 10 mM D-glucose as substrate at 75°C Caldicellulosiruptor saccharolyticus
0.04
-
 in 50 mM PIPES buffer (pH 7.5) containing 10 mM D-xylose as substrate at 75°C Caldicellulosiruptor saccharolyticus
0.06
-
 in 50 mM PIPES buffer (pH 7.5) containing 10 mM D-lyxose as substrate at 75°C Caldicellulosiruptor saccharolyticus
0.15
-
 in 50 mM PIPES buffer (pH 7.5) containing 10 mM D-mannose as substrate at 75°C Caldicellulosiruptor saccharolyticus
3.6
-
 in 50 mM PIPES buffer (pH 7.5) containing 10 mM cellobiose as substrate at 75°C Caldicellulosiruptor saccharolyticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose the enzyme activity is approximately 100fold higher for cellobiose than for D-glucose Caldicellulosiruptor saccharolyticus 4-O-beta-D-glucopyranosyl-D-mannose
-
 ?
D-fructose
-
 Caldicellulosiruptor saccharolyticus D-glucose + D-mannose
-
 ?
D-glucose
-
 Caldicellulosiruptor saccharolyticus D-mannose
-
 ?
D-lyxose
-
 Caldicellulosiruptor saccharolyticus D-xylose
-
 ?
D-mannose
-
 Caldicellulosiruptor saccharolyticus D-glucose
-
 ?
D-xylose
-
 Caldicellulosiruptor saccharolyticus D-lyxose
-
 ?
D-xylulose low activity Caldicellulosiruptor saccharolyticus D-xylose + D-lyxose
-
 ?
L-allose low activity Caldicellulosiruptor saccharolyticus L-altrose
-
 ?
L-altrose
-
 Caldicellulosiruptor saccharolyticus L-allose
-
 ?
L-arabinose low activity Caldicellulosiruptor saccharolyticus L-ribose
-
 ?
L-gulose low activity Caldicellulosiruptor saccharolyticus L-idose
-
 ?
L-idose
-
 Caldicellulosiruptor saccharolyticus L-gulose
-
 ?
L-psicose low activity Caldicellulosiruptor saccharolyticus L-allose + L-altrose
-
 ?
L-ribulose low activity Caldicellulosiruptor saccharolyticus L-arabinose + L-ribose
-
 ?
L-sorbose low activity Caldicellulosiruptor saccharolyticus L-gulose + L-idose
-
 ?
mannose the enzyme exhibits the highest isomerization activity for mannose among monosaccharides Caldicellulosiruptor saccharolyticus D-glucose + D-fructose
-
 ?
additional information the enzyme is not active for N-acyl-D-glucosamine Caldicellulosiruptor saccharolyticus ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 47000, SDS-PAGE Caldicellulosiruptor saccharolyticus
monomer 1 * 47340, calculated from amino acid sequence Caldicellulosiruptor saccharolyticus

Synonyms

Synonyms Comment Organism
cellobiose 2-epimerase
-
 Caldicellulosiruptor saccharolyticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
-
 Caldicellulosiruptor saccharolyticus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60 80 the half-lives of the enzyme at 60°C, 65°C, 70°C, 75°C, and 80°C are 142, 71, 35, 18, and 4.6 h, respectively Caldicellulosiruptor saccharolyticus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.27
-
 D-glucose in 50 mM PIPES buffer (pH 7.5), at 75°C Caldicellulosiruptor saccharolyticus
0.74
-
 D-mannose in 50 mM PIPES buffer (pH 7.5), at 75°C Caldicellulosiruptor saccharolyticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Caldicellulosiruptor saccharolyticus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0038
-
 D-glucose in 50 mM PIPES buffer (pH 7.5), at 75°C Caldicellulosiruptor saccharolyticus
0.0143
-
 D-mannose in 50 mM PIPES buffer (pH 7.5), at 75°C Caldicellulosiruptor saccharolyticus