Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bacteroides fragilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.7 | 5 | cellobiose | recombinant enzyme, 100 mM sodium phosphate, 20 mM cellobiose, pH 7.5, 30°C | Bacteroides fragilis | |
6.56 | - |
lactose | recombinant enzyme, 100 mM sodium phosphate, 20 mM lactose, pH 7.5, 30°C | Bacteroides fragilis | |
13.8 | - |
cellobiose | recombinant enzyme, 100 mM sodium phosphate, 20 mM cellobiose, pH 7.5, 30°C | Ruminococcus albus | |
33 | - |
lactose | recombinant enzyme, 100 mM sodium phosphate, 20 mM lactose, pH 7.5, 30°C | Ruminococcus albus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41100 | - |
determined by SDS-PAGE, the mass discrepancy putatively results from partial hydrolysis at the C-terminus during expression or in the purification steps | Bacteroides fragilis |
45780 | - |
calculated by amino acid sequence, confirmed by SDS-PAGE | Bacteroides fragilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-mannobiose | Bacteroides fragilis | - |
4-O-beta-D-mannopyranosyl-D-glucose | putative product | r | |
cellobiose | Ruminococcus albus | - |
4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
cellobiose | Bacteroides fragilis | - |
4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
globotriose | Bacteroides fragilis | - |
O-alpha-D-galactopyranosyl-(1,4)-O-beta-D-galactopyranosyl-(1,4)-D-mannose | putative product | r | |
lactose | Ruminococcus albus | - |
4-O-beta-D-galactopyranosyl-D-mannose | - |
r | |
lactose | Bacteroides fragilis | - |
4-O-beta-D-galactopyranosyl-D-mannose | - |
r | |
additional information | Bacteroides fragilis | recombinant enzyme catalyzes a hydroxyl stereoisomerism at the C2-positions of the reducing terminal glucose and at the mannose moiety of cello-oligosaccharides: lactose, beta-mannobiose and globotriose | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacteroides fragilis | Q5LH66 | - |
- |
Ruminococcus albus | P0DKY4 | - |
- |
Purification (Comment) | Organism |
---|---|
by anion exchange chromatography, enzyme is further purified on a hydroxyapatite column | Bacteroides fragilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
28.4 | - |
recombinant enzyme, substrate is lactose | Ruminococcus albus |
29.9 | - |
crude extract after expression in Escherichia coli cells | Bacteroides fragilis |
51 | - |
recombinant enzyme, substrate is cellobiose | Ruminococcus albus |
58.3 | - |
recombinant enzyme after first purification step on a DEAE Sepharose FF column | Bacteroides fragilis |
61.8 | - |
recombinant enzyme after second purification step on a hydroxyapatite column, substrate is cellobiose | Bacteroides fragilis |
79.7 | - |
recombinant enzyme after second purification step on a hydroxyapatite column, substrate is lactose | Bacteroides fragilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-mannobiose | - |
Bacteroides fragilis | 4-O-beta-D-mannopyranosyl-D-glucose | putative product | r | |
cellobiose | - |
Ruminococcus albus | 4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
cellobiose | - |
Bacteroides fragilis | 4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
globotriose | - |
Bacteroides fragilis | O-alpha-D-galactopyranosyl-(1,4)-O-beta-D-galactopyranosyl-(1,4)-D-mannose | putative product | r | |
lactose | - |
Ruminococcus albus | 4-O-beta-D-galactopyranosyl-D-mannose | - |
r | |
lactose | - |
Bacteroides fragilis | 4-O-beta-D-galactopyranosyl-D-mannose | - |
r | |
additional information | recombinant enzyme catalyzes a hydroxyl stereoisomerism at the C2-positions of the reducing terminal glucose and at the mannose moiety of cello-oligosaccharides: lactose, beta-mannobiose and globotriose | Bacteroides fragilis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cellobiose 2-epimerase | - |
Ruminococcus albus |
cellobiose 2-epimerase | - |
Bacteroides fragilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Bacteroides fragilis |
45 | - |
optimum | Bacteroides fragilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
52.1 | - |
lactose | recombinant enzyme, 100 mM sodium phosphate, 20 mM lactose, pH 7.5, 30°C | Ruminococcus albus | |
63.8 | - |
cellobiose | recombinant enzyme, 100 mM sodium phosphate, 20 mM cellobiose, pH 7.5, 30°C | Ruminococcus albus | |
67.6 | - |
cellobiose | recombinant enzyme, 100 mM sodium phosphate, 20 mM cellobiose, pH 7.5, 30°C | Bacteroides fragilis | |
79.5 | - |
lactose | recombinant enzyme, 100 mM sodium phosphate, 20 mM lactose, pH 7.5, 30°C | Bacteroides fragilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Bacteroides fragilis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.58 | - |
lactose | recombinant enzyme, 100 mM sodium phosphate, 20 mM lactose, pH 7.5, 30°C | Ruminococcus albus | |
4.62 | - |
cellobiose | recombinant enzyme, 100 mM sodium phosphate, 20 mM cellobiose, pH 7.5, 30°C | Ruminococcus albus | |
12.1 | - |
lactose | recombinant enzyme, 100 mM sodium phosphate, 20 mM lactose, pH 7.5, 30°C | Bacteroides fragilis | |
18.1 | - |
cellobiose | recombinant enzyme, 100 mM sodium phosphate, 20 mM cellobiose, pH 7.5, 30°C | Bacteroides fragilis |