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Literature summary for 5.1.3.11 extracted from

  • Ito, S.; Taguchi, H.; Hamada, S.; Kawauchi, S.; Ito, H.; Senoura, T.; Watanabe, J.; Nishimukai, M.; Ito, S.; Matsui, H.
    Enzymatic properties of cellobiose 2-epimerase from Ruminococcus albus and the synthesis of rare oligosaccharides by the enzyme (2008), Appl. Microbiol. Biotechnol., 79, 433-441.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Ruminococcus albus

Inhibitors

Inhibitors Comment Organism Structure
4-chloromercuribenzoate
-
Ruminococcus albus
Ag+
-
Ruminococcus albus
Al3+
-
Ruminococcus albus
Co2+
-
Ruminococcus albus
Cu2+
-
Ruminococcus albus
Fe3+
-
Ruminococcus albus
iodoacetate
-
Ruminococcus albus
N-bromosuccinimide
-
Ruminococcus albus
Pb2+
-
Ruminococcus albus
Zn2+
-
Ruminococcus albus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
13.8
-
cellobiose pH 7.5, 30°C Ruminococcus albus
33
-
lactose pH 7.5, 30°C Ruminococcus albus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43100
-
1 * 43100, SDS-PAGE Ruminococcus albus
44000
-
gel filtration Ruminococcus albus

Organism

Organism UniProt Comment Textmining
Ruminococcus albus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Ruminococcus albus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
28.4
-
substrate lactose, pH 7.5, 30°C Ruminococcus albus
51
-
substrate cellobiose, pH 7.5, 30°C Ruminococcus albus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose
-
Ruminococcus albus 4-O-beta-D-glucopyranosyl-D-mannose
-
?
lactose
-
Ruminococcus albus 4-O-beta-D-galactopyranosyl-D-mannose i.e. epilactose ?
additional information no substrates: N-acetyl-D-glucosamine, uridine 5'-diphosphate-glucose, D-glucose 6-phosphate, maltose, sophorose, laminaribiose, and gentiobiose Ruminococcus albus ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 43100, SDS-PAGE Ruminococcus albus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Ruminococcus albus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
52.1
-
lactose pH 7.5, 30°C Ruminococcus albus
63.8
-
cellobiose pH 7.5, 30°C Ruminococcus albus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Ruminococcus albus