Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | pyridoxal 5'-phosphate is not involved in the reaction | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Pseudomonas putida | |
2,2'-dipyridyl | - |
Pseudomonas putida | |
Acriflavin | - |
Pseudomonas putida | |
AgNO3 | - |
Pseudomonas putida | |
Atebrin | - |
Pseudomonas putida | |
CdCl2 | - |
Pseudomonas putida | |
CuSO4 | - |
Pseudomonas putida | |
HgCl2 | - |
Pseudomonas putida | |
hydrazine | - |
Pseudomonas putida | |
hydroxylamine | - |
Pseudomonas putida | |
iodoacetate | - |
Pseudomonas putida | |
KCN | - |
Pseudomonas putida | |
NaAsO2 | - |
Pseudomonas putida | |
NEM | - |
Pseudomonas putida | |
p-chloromercuribenzoate | - |
Pseudomonas putida | |
Semicarbazide | - |
Pseudomonas putida | |
ZnCl2 | - |
Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
22 | - |
hydroxy-L-Pro | - |
Pseudomonas putida | |
53 | - |
allohydroxy-L-Pro | - |
Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
17930 | - |
low speed sedimentation without reaching equilibrium | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Hydroxy-L-Pro | Pseudomonas putida | trans-hydroxy-L-Pro or cis-hydroxy-L-Pro, initial reaction of the pathway of hydroxyproline metabolism | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
trans-4-Hydroxy-L-proline = cis-4-hydroxy-D-proline | one hydrogen in the substrate is labilized during the reaction | Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
100 | - |
- |
Pseudomonas putida |
Storage Stability | Organism |
---|---|
-15°C, concentrated purified enzyme is stable for at least several weeks | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Allohydroxy-D-Pro | - |
Pseudomonas putida | Allohydroxy-L-Pro | - |
? | |
D-Ala | at 1-2% of the activity relative to allo-D-hydroxy-proline epimerization | Pseudomonas putida | L-Ala | - |
? | |
Hydroxy-L-Pro | trans-hydroxy-L-Pro or cis-hydroxy-L-Pro, initial reaction of the pathway of hydroxyproline metabolism | Pseudomonas putida | ? | - |
? | |
trans-4-hydroxy-L-Pro | - |
Pseudomonas putida | cis-4-hydroxy-D-Pro | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | 8 | allohydroxy-L-proline | Pseudomonas putida |
8 | - |
hydroxy-L-proline | Pseudomonas putida |