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Literature summary for 5.1.1.4 extracted from
- Studies on the enzymic reduction of amino acids. II. Purification and properties of a D-proline reductase and a proline racemase from Clostridium sticklandii (1957), J. Biol. Chem., 228, 983-997.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | activates | Acetoanaerobium sticklandii |  |
| additional information | pyridoxal 5'-phosphate is not involved in racemization | Acetoanaerobium sticklandii | |
| SH compounds | activate, e.g. 2-mercaptoethanol, 1,3-dimercaptoethanol | Acetoanaerobium sticklandii | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| lipoic acid | weak | Acetoanaerobium sticklandii | |
| reduced glutathione | weak | Acetoanaerobium sticklandii | |
| thioglycolate | weak | Acetoanaerobium sticklandii | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 37 mM, 25% inhibition | Acetoanaerobium sticklandii | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acetoanaerobium sticklandii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Acetoanaerobium sticklandii |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| L-proline = D-proline | racemization is accompanied by deuterium incorporation from the solvent into the alpha position of Pro, participation of two equivalent hydrogen acceptor sites | Acetoanaerobium sticklandii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.8 | - |
- |
Acetoanaerobium sticklandii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-Pro | - |
Acetoanaerobium sticklandii | D-Pro | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 24 | 37 | at 37°C the activity is 35-40% greater than at 24°C | Acetoanaerobium sticklandii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.6 | 8.1 | pH 5.5: no detectable activity, rapid fall of activity below pH 6.5, activity is relatively stable between pH 6.7 and 8.1 | Acetoanaerobium sticklandii |
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Release 2023.1 (January 2023)
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