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Literature summary for 5.1.1.18 extracted from

  • Mustafa, A.K.; Kumar, M.; Selvakumar, B.; Ho, G.P.; Ehmsen, J.T.; Barrow, R.K.; Amzel, L.M.; Snyder, S.H.
    Nitric oxide S-nitrosylates serine racemase, mediating feedback inhibition of D-serine formation (2007), Proc. Natl. Acad. Sci. USA, 104, 2950-2955.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.1.1.18

Activating Compound

Activating Compound Comment Organism Structure
ATP required Mus musculus

Cloned(Commentary)

Cloned (Comment) Organism
expression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Mus musculus

Protein Variants

Protein Variants Comment Organism
C113S site-directed mutagenesis, the mutant is resistant to regulation by nitrosylation by NO Mus musculus
K51A site-directed mutagenesis, the K51A mutant shows substantially less ATP binding and reduced activity compared to the wild-type enzyme Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
nitric oxide nitric oxide physiologically S-nitrosylates the enzyme at Cys113, mediating feedback inhibition of D-serine, which is enhanced by the NMDA receptor through activation of neuronal nitric oxide synthase Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-serine Mus musculus D-serine is a coagonist with glutamate at NMDA receptors, postsynaptic stimulation of nitric-oxide formation feeds back to presynaptic cells to S-nitrosylate SR and decrease D-serine availability to postsynaptic NMDA receptors, enzyme regulation, mechanism, overview D-serine
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus Q9QZX7
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) using Talon resin chromatography Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
astrocyte
-
 Mus musculus
-
brain
-
 Mus musculus
-
neuron
-
 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-serine
-
 Mus musculus D-serine
-
 ?
L-serine D-serine is a coagonist with glutamate at NMDA receptors, postsynaptic stimulation of nitric-oxide formation feeds back to presynaptic cells to S-nitrosylate SR and decrease D-serine availability to postsynaptic NMDA receptors, enzyme regulation, mechanism, overview Mus musculus D-serine
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7
-
 assay at Mus musculus