Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ATP | activates, synergy between Mg2+ and ATP on activity | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
hydroxylamine | - |
Arabidopsis thaliana | |
additional information | no inhibition by EDTA of the racemase activity | Arabidopsis thaliana | |
Ni2+ | - |
Arabidopsis thaliana | |
Zn2+ | - |
Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of the bifunctional enzyme, overview | Arabidopsis thaliana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates optimally 3.6fold at 1 mM | Arabidopsis thaliana | |
Fe2+ | activates 1.4fold at 1 mM | Arabidopsis thaliana | |
Mg2+ | activates 2.9fold at 1 mM | Arabidopsis thaliana | |
Mn2+ | activates 3.0fold at 1 mM, synergy between Mg2+ and ATP on activity | Arabidopsis thaliana | |
additional information | the enzyme requires divalent cations, effects of metal ions on the enzyme's dehydration activity, overview | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
2 * 35000, about, recombinant enzyme, SDS-PAGE and amino acid sequence calculation | Arabidopsis thaliana |
60000 | - |
recombinant enzyme, gel filtration | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine | Arabidopsis thaliana | - |
D-serine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q2PGG3 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | ubiquitous tissue expression of the enzyme | Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanine | 13% of the activity with L-serine | Arabidopsis thaliana | D-alanine | - |
? | |
L-arginine | 7% of the activity with L-serine | Arabidopsis thaliana | D-arginine | - |
? | |
L-glutamine | 4.5% of the activity with L-serine | Arabidopsis thaliana | D-glutamine | - |
? | |
L-serine | - |
Arabidopsis thaliana | D-serine | - |
? | |
L-serine | highly specific for | Arabidopsis thaliana | D-serine | - |
? | |
additional information | the bifunctional enzyme catalyzes not only serine racemization but also dehydration of serine to pyruvate | Arabidopsis thaliana | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 35000, about, recombinant enzyme, SDS-PAGE and amino acid sequence calculation | Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Arabidopsis thaliana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 9.5 | high activity within this range | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | required, bound through a Schiff base, conserved binding site structure involving Lys59, Phe58, Gly238, Ser314, and Asn86, overview | Arabidopsis thaliana |