Activating Compound | Comment | Organism | Structure |
---|---|---|---|
SH-protecting reagents | e.g., 2-mercaptoethanol and dithiothreitol increase activity 2fold | Streptococcus thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
2 * 28000, SDS-PAGE | Streptococcus thermophilus |
60000 | - |
gel filtration | Streptococcus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterococcus faecalis | - |
strain ATCC 9790, strain IAM 10065, no activity in strain IAM 10067 | - |
Enterococcus faecium | - |
- |
- |
Lacticaseibacillus casei | - |
- |
- |
Lactobacillus delbrueckii | - |
- |
- |
Lactobacillus delbrueckii subsp. bulgaricus | - |
- |
- |
Lactococcus lactis | - |
- |
- |
Levilactobacillus brevis | - |
- |
- |
no activity in Bifidobacterium bifidum | - |
- |
- |
no activity in Leuconostoc mesenteroides | - |
- |
- |
no activity in Pediococcus pentosaceus | - |
- |
- |
Streptococcus thermophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptococcus thermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
26.8 | - |
- |
Streptococcus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Ala | no racemase activity | Streptococcus thermophilus | D-Ala | - |
? | |
L-Asp | - |
Lactococcus lactis | D-Asp | - |
? | |
L-Asp | - |
Enterococcus faecalis | D-Asp | - |
? | |
L-Asp | - |
Lacticaseibacillus casei | D-Asp | - |
? | |
L-Asp | - |
Enterococcus faecium | D-Asp | - |
? | |
L-Asp | - |
Streptococcus thermophilus | D-Asp | - |
? | |
L-Asp | - |
Levilactobacillus brevis | D-Asp | - |
? | |
L-Asp | - |
Lactobacillus delbrueckii subsp. bulgaricus | D-Asp | - |
? | |
L-Asp | - |
Lactobacillus delbrueckii | D-Asp | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 28000, SDS-PAGE | Streptococcus thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Lactococcus lactis |
37 | - |
- |
Streptococcus thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
1 h, 50 mM potassium phosphate buffer, stable | Streptococcus thermophilus |
37 | - |
2 h, complete inactivation | Lactococcus lactis |
50 | - |
1 h, about 60% loss of activity, pH 7.2 | Streptococcus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
- |
Streptococcus thermophilus |
8 | - |
- |
Streptococcus thermophilus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7.5 | stable | Streptococcus thermophilus |