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Literature summary for 5.1.1.13 extracted from

  • Okada, H.; Yohda, M.; Giga-Hama, Y.; Ueno, Y.; Ohdo, S.; Kumagai, H.
    Distribution and purification of asparate racemase in lactic acid bacteria (1991), Biochim. Biophys. Acta, 1078, 377-382.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
SH-protecting reagents e.g., 2-mercaptoethanol and dithiothreitol increase activity 2fold Streptococcus thermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
2 * 28000, SDS-PAGE Streptococcus thermophilus
60000
-
gel filtration Streptococcus thermophilus

Organism

Organism UniProt Comment Textmining
Enterococcus faecalis
-
strain ATCC 9790, strain IAM 10065, no activity in strain IAM 10067
-
Enterococcus faecium
-
-
-
Lacticaseibacillus casei
-
-
-
Lactobacillus delbrueckii
-
-
-
Lactobacillus delbrueckii subsp. bulgaricus
-
-
-
Lactococcus lactis
-
-
-
Levilactobacillus brevis
-
-
-
no activity in Bifidobacterium bifidum
-
-
-
no activity in Leuconostoc mesenteroides
-
-
-
no activity in Pediococcus pentosaceus
-
-
-
Streptococcus thermophilus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptococcus thermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
26.8
-
-
Streptococcus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Ala no racemase activity Streptococcus thermophilus D-Ala
-
?
L-Asp
-
Lactococcus lactis D-Asp
-
?
L-Asp
-
Enterococcus faecalis D-Asp
-
?
L-Asp
-
Lacticaseibacillus casei D-Asp
-
?
L-Asp
-
Enterococcus faecium D-Asp
-
?
L-Asp
-
Streptococcus thermophilus D-Asp
-
?
L-Asp
-
Levilactobacillus brevis D-Asp
-
?
L-Asp
-
Lactobacillus delbrueckii subsp. bulgaricus D-Asp
-
?
L-Asp
-
Lactobacillus delbrueckii D-Asp
-
?

Subunits

Subunits Comment Organism
dimer 2 * 28000, SDS-PAGE Streptococcus thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Lactococcus lactis
37
-
-
Streptococcus thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
1 h, 50 mM potassium phosphate buffer, stable Streptococcus thermophilus
37
-
2 h, complete inactivation Lactococcus lactis
50
-
1 h, about 60% loss of activity, pH 7.2 Streptococcus thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
-
Streptococcus thermophilus
8
-
-
Streptococcus thermophilus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 7.5 stable Streptococcus thermophilus