Literature summary for 5.1.1.12 extracted from

Fonknechten, N.; Perret, A.; Perchat, N.; Tricot, S.; Lechaplais, C.; Vallenet, D.; Vergne, C.; Zaparucha, A.; Le Paslier, D.; Weissenbach, J.; Salanoubat, M.
A conserved gene cluster rules anaerobic oxidative degradation of L-ornithine (2009), J. Bacteriol., 191, 3162-3167.

Search for more literature for 5.1.1.12

Cloned(Commentary)

Cloned (Comment)	Organism
expressed His-tagged recombinant protein	Acetoanaerobium sticklandii

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.52	-	L-ornithine	-	Acetoanaerobium sticklandii

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
21500	-	2 * 21500, gel filtration	Acetoanaerobium sticklandii
43000	-	SDS-PAGE	Acetoanaerobium sticklandii

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-Ornithine	Acetoanaerobium sticklandii	-	D-Ornithine	-	r

Organism

Organism	UniProt	Comment	Textmining
Acetoanaerobium sticklandii	C1FW08	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Acetoanaerobium sticklandii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-alanine	conversion is lower than 10% compared to conversion of L-ornithine	Acetoanaerobium sticklandii	L-alanine	-	r
L-asparagine	conversion is lower than 10% compared to conversion of L-ornithine	Acetoanaerobium sticklandii	D-asparagine	-	r
L-Ornithine	-	Acetoanaerobium sticklandii	D-Ornithine	-	r
L-serine	conversion is 10% compared to conversion of L-ornithine	Acetoanaerobium sticklandii	D-serine	-	r

Subunits

Subunits	Comment	Organism
homodimer	2 * 21500, gel filtration	Acetoanaerobium sticklandii

Synonyms

Synonyms	Comment	Organism
L-ornithine racemase	-	Acetoanaerobium sticklandii
or-5	-	Acetoanaerobium sticklandii

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1660	-	L-ornithine	-	Acetoanaerobium sticklandii

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	-	Acetoanaerobium sticklandii

General Information

General Information	Comment	Organism
metabolism	catalyzes the first step in the ornithine fermentation pathway	Acetoanaerobium sticklandii

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
3192	-	L-ornithine	-	Acetoanaerobium sticklandii

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Release 2023.1 (January 2023)