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Literature summary for 5.1.1.10 extracted from

  • Pozo-Dengra, J.; Martínez-Gómez, A.; Martínez-Rodríguez, S.; Clemente-Jiménez, J.; Rodríguez-Vico, F.; Heras-Vázquez, F.
    Racemization study on different N-acetylamino acids by a recombinant N-succinylamino acid racemase from Geobacillus kaustophilus CECT4264 (2009), Process Biochem., 44, 835-841.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
over-expressed in Escherichia coli Geobacillus kaustophilus

Inhibitors

Inhibitors Comment Organism Structure
8-hydroxy-5-quinolinesulfonic acid
-
Geobacillus kaustophilus
beta-mercaptoethanol
-
Geobacillus kaustophilus
dithiothreitol
-
Geobacillus kaustophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
N-succinyl-D-phenylalanine
-
Geobacillus kaustophilus
0.12
-
N-succinyl-L-alanine
-
Geobacillus kaustophilus
0.13
-
N-succinyl-L-phenylalanine
-
Geobacillus kaustophilus
0.13
-
N-succinyl-D-alanine
-
Geobacillus kaustophilus
2
-
N-acetyl-L-tryptophan
-
Geobacillus kaustophilus
2
-
N-acetyl-D-tryptophan
-
Geobacillus kaustophilus
2
-
N-carbamoyl-D-methionine
-
Geobacillus kaustophilus
2 3 N-acetyl-D-phenylalanine
-
Geobacillus kaustophilus
5
-
N-carbamoyl-L-methionine
-
Geobacillus kaustophilus
7
-
N-acetyl-D-methionine
-
Geobacillus kaustophilus
8
-
N-acetyl-L-methionine
-
Geobacillus kaustophilus
17
-
N-acetyl-D-alanine
-
Geobacillus kaustophilus
18
-
N-acetyl-D-asparagine
-
Geobacillus kaustophilus
27
-
N-acetyl-L-asparagine
-
Geobacillus kaustophilus
41
-
N-acetyl-L-alanine
-
Geobacillus kaustophilus
43
-
N-acetyl-L-phenylalanine
-
Geobacillus kaustophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ enhances activity, highest activity with Co2+ Geobacillus kaustophilus
Mn2+ enhances activity Geobacillus kaustophilus
Ni2+ enhances activity Geobacillus kaustophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
SDS-PAGE Geobacillus kaustophilus
150000
-
gel filtration, tetramer Geobacillus kaustophilus

Organism

Organism UniProt Comment Textmining
Geobacillus kaustophilus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purified in a one-step procedure by immobilized cobalt affinity chromatography Geobacillus kaustophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-D-alanine
-
Geobacillus kaustophilus N-acetyl-L-alanine
-
r
N-acetyl-D-asparagine
-
Geobacillus kaustophilus N-acetyl-L-asparagine
-
r
N-acetyl-D-methionine
-
Geobacillus kaustophilus N-acetyl-L-methionine
-
r
N-acetyl-D-phenylalanine
-
Geobacillus kaustophilus N-acetyl-L-phenylalanine
-
r
N-acetyl-D-tryptophan
-
Geobacillus kaustophilus N-acetyl-L-tryptophan
-
r
N-carbamoyl-L-methionine
-
Geobacillus kaustophilus N-carbamoyl-D-methionine
-
r
N-succinyl-L-alanine
-
Geobacillus kaustophilus N-succinyl-D-alanine
-
r
N-succinyl-L-phenylalanine
-
Geobacillus kaustophilus N-succinyl-D-phenylalanine
-
r

Subunits

Subunits Comment Organism
homotetramer 4 * 43000, SDS-PAGE Geobacillus kaustophilus

Synonyms

Synonyms Comment Organism
GkNSAAR
-
Geobacillus kaustophilus
N-succinylamino acid racemase
-
Geobacillus kaustophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
Geobacillus kaustophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
GkNSAAR shows a gradual loss of activity at preincubation temperatures over 60°C Geobacillus kaustophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.06
-
N-acetyl-L-asparagine
-
Geobacillus kaustophilus
0.07
-
N-acetyl-D-asparagine
-
Geobacillus kaustophilus
0.09
-
N-acetyl-D-tryptophan
-
Geobacillus kaustophilus
0.15
-
N-acetyl-L-tryptophan
-
Geobacillus kaustophilus
0.8
-
N-acetyl-D-alanine
-
Geobacillus kaustophilus
2
-
N-acetyl-L-alanine
-
Geobacillus kaustophilus
2
-
N-carbamoyl-L-methionine
-
Geobacillus kaustophilus
2
-
N-carbamoyl-D-methionine
-
Geobacillus kaustophilus
2
-
N-succinyl-D-phenylalanine
-
Geobacillus kaustophilus
5
-
N-succinyl-L-phenylalanine
-
Geobacillus kaustophilus
10
-
N-acetyl-D-phenylalanine
-
Geobacillus kaustophilus
15
-
N-succinyl-D-alanine
-
Geobacillus kaustophilus
16
-
N-acetyl-L-phenylalanine
-
Geobacillus kaustophilus
20
-
N-acetyl-D-methionine
-
Geobacillus kaustophilus
22
-
N-acetyl-L-methionine
-
Geobacillus kaustophilus
43
-
N-succinyl-L-alanine
-
Geobacillus kaustophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Geobacillus kaustophilus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.002
-
N-acetyl-L-asparagine
-
Geobacillus kaustophilus
0.004
-
N-acetyl-D-asparagine
-
Geobacillus kaustophilus
0.039
-
N-acetyl-L-alanine
-
Geobacillus kaustophilus
0.047
-
N-acetyl-D-alanine
-
Geobacillus kaustophilus
0.06
-
N-acetyl-L-tryptophan
-
Geobacillus kaustophilus
0.061
-
N-acetyl-D-tryptophan
-
Geobacillus kaustophilus
0.37
-
N-acetyl-L-phenylalanine
-
Geobacillus kaustophilus
0.467
-
N-acetyl-D-phenylalanine
-
Geobacillus kaustophilus
1.1
-
N-carbamoyl-L-methionine
-
Geobacillus kaustophilus
1.2
-
N-carbamoyl-D-methionine
-
Geobacillus kaustophilus
2.8
-
N-acetyl-L-methionine
-
Geobacillus kaustophilus
3
-
N-acetyl-D-methionine
-
Geobacillus kaustophilus
35.1
-
N-succinyl-L-phenylalanine
-
Geobacillus kaustophilus
38.1
-
N-succinyl-D-phenylalanine
-
Geobacillus kaustophilus
119
-
N-succinyl-D-alanine
-
Geobacillus kaustophilus
352
-
N-succinyl-L-alanine
-
Geobacillus kaustophilus