Cloned (Comment) | Organism |
---|---|
over-expressed in Escherichia coli | Geobacillus kaustophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
8-hydroxy-5-quinolinesulfonic acid | - |
Geobacillus kaustophilus | |
beta-mercaptoethanol | - |
Geobacillus kaustophilus | |
dithiothreitol | - |
Geobacillus kaustophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
N-succinyl-D-phenylalanine | - |
Geobacillus kaustophilus | |
0.12 | - |
N-succinyl-L-alanine | - |
Geobacillus kaustophilus | |
0.13 | - |
N-succinyl-L-phenylalanine | - |
Geobacillus kaustophilus | |
0.13 | - |
N-succinyl-D-alanine | - |
Geobacillus kaustophilus | |
2 | - |
N-acetyl-L-tryptophan | - |
Geobacillus kaustophilus | |
2 | - |
N-acetyl-D-tryptophan | - |
Geobacillus kaustophilus | |
2 | - |
N-carbamoyl-D-methionine | - |
Geobacillus kaustophilus | |
2 | 3 | N-acetyl-D-phenylalanine | - |
Geobacillus kaustophilus | |
5 | - |
N-carbamoyl-L-methionine | - |
Geobacillus kaustophilus | |
7 | - |
N-acetyl-D-methionine | - |
Geobacillus kaustophilus | |
8 | - |
N-acetyl-L-methionine | - |
Geobacillus kaustophilus | |
17 | - |
N-acetyl-D-alanine | - |
Geobacillus kaustophilus | |
18 | - |
N-acetyl-D-asparagine | - |
Geobacillus kaustophilus | |
27 | - |
N-acetyl-L-asparagine | - |
Geobacillus kaustophilus | |
41 | - |
N-acetyl-L-alanine | - |
Geobacillus kaustophilus | |
43 | - |
N-acetyl-L-phenylalanine | - |
Geobacillus kaustophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | enhances activity, highest activity with Co2+ | Geobacillus kaustophilus | |
Mn2+ | enhances activity | Geobacillus kaustophilus | |
Ni2+ | enhances activity | Geobacillus kaustophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
SDS-PAGE | Geobacillus kaustophilus |
150000 | - |
gel filtration, tetramer | Geobacillus kaustophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus kaustophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purified in a one-step procedure by immobilized cobalt affinity chromatography | Geobacillus kaustophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-D-alanine | - |
Geobacillus kaustophilus | N-acetyl-L-alanine | - |
r | |
N-acetyl-D-asparagine | - |
Geobacillus kaustophilus | N-acetyl-L-asparagine | - |
r | |
N-acetyl-D-methionine | - |
Geobacillus kaustophilus | N-acetyl-L-methionine | - |
r | |
N-acetyl-D-phenylalanine | - |
Geobacillus kaustophilus | N-acetyl-L-phenylalanine | - |
r | |
N-acetyl-D-tryptophan | - |
Geobacillus kaustophilus | N-acetyl-L-tryptophan | - |
r | |
N-carbamoyl-L-methionine | - |
Geobacillus kaustophilus | N-carbamoyl-D-methionine | - |
r | |
N-succinyl-L-alanine | - |
Geobacillus kaustophilus | N-succinyl-D-alanine | - |
r | |
N-succinyl-L-phenylalanine | - |
Geobacillus kaustophilus | N-succinyl-D-phenylalanine | - |
r |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 43000, SDS-PAGE | Geobacillus kaustophilus |
Synonyms | Comment | Organism |
---|---|---|
GkNSAAR | - |
Geobacillus kaustophilus |
N-succinylamino acid racemase | - |
Geobacillus kaustophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Geobacillus kaustophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
GkNSAAR shows a gradual loss of activity at preincubation temperatures over 60°C | Geobacillus kaustophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
N-acetyl-L-asparagine | - |
Geobacillus kaustophilus | |
0.07 | - |
N-acetyl-D-asparagine | - |
Geobacillus kaustophilus | |
0.09 | - |
N-acetyl-D-tryptophan | - |
Geobacillus kaustophilus | |
0.15 | - |
N-acetyl-L-tryptophan | - |
Geobacillus kaustophilus | |
0.8 | - |
N-acetyl-D-alanine | - |
Geobacillus kaustophilus | |
2 | - |
N-acetyl-L-alanine | - |
Geobacillus kaustophilus | |
2 | - |
N-carbamoyl-L-methionine | - |
Geobacillus kaustophilus | |
2 | - |
N-carbamoyl-D-methionine | - |
Geobacillus kaustophilus | |
2 | - |
N-succinyl-D-phenylalanine | - |
Geobacillus kaustophilus | |
5 | - |
N-succinyl-L-phenylalanine | - |
Geobacillus kaustophilus | |
10 | - |
N-acetyl-D-phenylalanine | - |
Geobacillus kaustophilus | |
15 | - |
N-succinyl-D-alanine | - |
Geobacillus kaustophilus | |
16 | - |
N-acetyl-L-phenylalanine | - |
Geobacillus kaustophilus | |
20 | - |
N-acetyl-D-methionine | - |
Geobacillus kaustophilus | |
22 | - |
N-acetyl-L-methionine | - |
Geobacillus kaustophilus | |
43 | - |
N-succinyl-L-alanine | - |
Geobacillus kaustophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Geobacillus kaustophilus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
N-acetyl-L-asparagine | - |
Geobacillus kaustophilus | |
0.004 | - |
N-acetyl-D-asparagine | - |
Geobacillus kaustophilus | |
0.039 | - |
N-acetyl-L-alanine | - |
Geobacillus kaustophilus | |
0.047 | - |
N-acetyl-D-alanine | - |
Geobacillus kaustophilus | |
0.06 | - |
N-acetyl-L-tryptophan | - |
Geobacillus kaustophilus | |
0.061 | - |
N-acetyl-D-tryptophan | - |
Geobacillus kaustophilus | |
0.37 | - |
N-acetyl-L-phenylalanine | - |
Geobacillus kaustophilus | |
0.467 | - |
N-acetyl-D-phenylalanine | - |
Geobacillus kaustophilus | |
1.1 | - |
N-carbamoyl-L-methionine | - |
Geobacillus kaustophilus | |
1.2 | - |
N-carbamoyl-D-methionine | - |
Geobacillus kaustophilus | |
2.8 | - |
N-acetyl-L-methionine | - |
Geobacillus kaustophilus | |
3 | - |
N-acetyl-D-methionine | - |
Geobacillus kaustophilus | |
35.1 | - |
N-succinyl-L-phenylalanine | - |
Geobacillus kaustophilus | |
38.1 | - |
N-succinyl-D-phenylalanine | - |
Geobacillus kaustophilus | |
119 | - |
N-succinyl-D-alanine | - |
Geobacillus kaustophilus | |
352 | - |
N-succinyl-L-alanine | - |
Geobacillus kaustophilus |