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Literature summary for 5.1.1.10 extracted from

  • Bechtold, M.; Makart, S.; Reiss, R.; Alder, P.; Panke, S.
    Model-based characterization of an amino acid racemase from Pseudomonas putida DSM 3263 for application in medium-constrained continuous processes (2007), Biotechnol. Bioeng., 98, 812-824.
    View publication on PubMed
Search for more literature for 5.1.1.10

Cloned(Commentary)

Cloned (Comment) Organism
amino acid racemase of Pseudomonas putida DSM 3263 is overexpressed in Escherichia coli and delivered cell free extract with easily sufficient activity (20–50 U/mg total protein) for application in an enzyme membrane reactor (EMR) setting Pseudomonas putida

General Stability

General Stability Organism
buffer without organic content provides excellent stability at moderate temperatures (20–35°C) while addition of 20% acetonitrile or methanol drastically reduces the half-life of the racemase Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information model-based calculations suggested that a methanol content of 10% and an acetonitrile content of 20% provide maximum productivity in enzyme membrane reactor (EMR) operations. However product concentrations are decreased in comparison to purely aqueous operation due to decreasing solubility of methionine with increasing organic solvent content. Pseudomonas putida
4.1
-
 L-methionine 100% aqueous Pseudomonas putida
4.8
-
 D-methionine 100% aqueous Pseudomonas putida
7.8
-
 L-methionine 10% methanol Pseudomonas putida
9
-
 D-methionine 10% methanol Pseudomonas putida
9.4
-
 L-methionine 20% methanol Pseudomonas putida
11.1
-
 D-methionine 10% acetonitril Pseudomonas putida
11.5
-
 L-methionine 10% acetonitril Pseudomonas putida
11.9
-
 D-methionine 40% acetonitril Pseudomonas putida
13.2
-
 L-methionine 20% acetonitril Pseudomonas putida
13.2
-
 D-methionine 20% acetonitril Pseudomonas putida
15.8
-
 D-methionine 20% methanol Pseudomonas putida
16.1
-
 L-methionine 40% acetonitril Pseudomonas putida
22.3
-
 L-methionine 40% methanol Pseudomonas putida
24.3
-
 D-methionine 40% methanol Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
 DSM 3263
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Methionine
-
 Pseudomonas putida D-Methionine
-
 r

Synonyms

Synonyms Comment Organism
amino-acid racemase
-
 Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
 assay at Pseudomonas putida