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Literature summary for 5.1.1.10 extracted from

  • Lim, Y.H.; Yokoigawa, K.; Esaki, N.; Soda, K.
    A new amino acid racemase with threonine alpha-epimerase activity from Pseudomonas putida: purification and characterization (1993), J. Bacteriol., 175, 4213-4217.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.1.1.10

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.42
-
 D-Lys
-
 Pseudomonas putida
0.55
-
 L-Lys
-
 Pseudomonas putida
9.8
-
 D-Thr
-
 Pseudomonas putida
31
-
 L-Thr
-
 Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
 2 * 41000, SDS-PAGE Pseudomonas putida
82000
-
 gel filtration Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
920
-
 L-Lys as substrate Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Arg 80% of the activity relative to L-Lys Pseudomonas putida L-Arg
-
 ?
D-Thr
-
 Pseudomonas putida L-Thr
-
 r
L-Ala 6% of the activity relative to L-Lys Pseudomonas putida D-Ala
-
 ?
L-Asn 12% of the activity relative to L-Lys Pseudomonas putida D-Asn
-
 ?
L-Citrulline 45% of the activity relative to L-Lys Pseudomonas putida D-Citrulline
-
 ?
L-Ethionine 83% of the activity relative to L-Lys Pseudomonas putida D-Ethionine
-
 ?
L-Glu 80% of the activity relative to L-Lys Pseudomonas putida D-Glu
-
 ?
L-His 6% of the activity relative to L-Lys Pseudomonas putida D-His
-
 ?
L-Homoarginine 80% of the activity relative to L-Lys Pseudomonas putida L-Homoarginine
-
 ?
L-Homocitrulline 45% of the activity relative to L-Lys Pseudomonas putida D-Homocitrulline
-
 ?
L-homoserine 22% of the activity relative to L-Lys Pseudomonas putida D-homoserine
-
 ?
L-Leu 10% of the activity relative to L-Lys Pseudomonas putida D-Leu
-
 ?
L-Lys
-
 Pseudomonas putida D-Lys
-
 ?
L-Met 66% of the activity relative to L-Lys Pseudomonas putida D-Met
-
 ?
L-Norleucine 45% of the activity relative to L-Lys Pseudomonas putida D-Norleucine
-
 ?
L-Orn 80% of the activity relative to L-Lys Pseudomonas putida D-Orn
-
 ?
L-Ser 10% of the activity relative to L-Lys Pseudomonas putida D-Ser
-
 ?
L-Thr 3% of the activity relative to L-Lys Pseudomonas putida L-Thr
-
 ?
N6-Acetyl-L-Lys 80% of the activity relative to L-Lys Pseudomonas putida N6-Acetyl-D-Lys
-
 ?
S-Methyl-L-Cys 3% of the activity relative to L-Lys Pseudomonas putida S-Methyl-D-Cys
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 41000, SDS-PAGE Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate holoenzyme contains 2 mol of pyridoxal 5'-phosphate per mol of enzyme Pseudomonas putida
pyridoxal 5'-phosphate contains 1 mol of pyridoxal 5'-phosphate per mol of subunit Pseudomonas putida