Crystallization (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-cycloserine | - |
Pseudomonas putida | |
hydroxylamine | - |
Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
30 | - |
D-Lys | - |
Pseudomonas putida | |
33 | - |
L-Leu | L-2-aminobutyrate | Pseudomonas putida | |
35 | - |
L-ethionine | - |
Pseudomonas putida | |
36 | - |
L-2-aminobutyrate | L-Ala | Pseudomonas putida | |
38 | - |
L-Met | - |
Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
708.4 | - |
- |
Pseudomonas putida |
Storage Stability | Organism |
---|---|
0-5°C, 0.01 M potassium phosphate buffer, pH 7.0, 2 mM pyridoxal 5'-phosphate, 1 month, stable | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-2,4-Diaminobutyrate | 10% of the activity relative to D-Lys | Pseudomonas putida | L-2,4-Diaminobutyrate | - |
? | |
D-Arg | 60% of the activity relative to D-Lys | Pseudomonas putida | L-Arg | - |
? | |
L-2-Aminobutyrate | 4.7% of the activity relative to D-Lys | Pseudomonas putida | D-2-Aminobutyrate | - |
? | |
L-Ala | 9% of the activity relative to D-Lys | Pseudomonas putida | D-Ala | - |
? | |
L-Citrulline | 16% of the activity relative to D-Lys | Pseudomonas putida | D-Citrulline | - |
? | |
L-Ethionine | 76% of the activity relative to D-Lys | Pseudomonas putida | D-Ethionine | - |
? | |
L-His | 1.8% of the activity relative to D-Lys | Pseudomonas putida | D-His | - |
? | |
L-Leu | 3% of the activity relative to D-Lys | Pseudomonas putida | D-Leu | - |
? | |
L-Lys | - |
Pseudomonas putida | D-Lys | - |
? | |
L-Met | 48% of the activity relative to D-Lys | Pseudomonas putida | D-Met | - |
? | |
L-Ser | 8.7% of the activity relative to D-Lys | Pseudomonas putida | D-Ser | - |
? | |
additional information | no activity with: D-Glu, L-Ile, L-Phe, L-Thr, L-Val | Pseudomonas putida | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | - |
Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | Km: 0.2 mM | Pseudomonas putida | |
pyridoxal 5'-phosphate | holoenzyme contains 2 mol of pyridoxal 5'-phosphate per mol of enzyme | Pseudomonas putida |