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Literature summary for 5.1.1.10 extracted from
- Crystalline amino acid racemase with low substrate specificity (1969), Biochem. Biophys. Res. Commun., 35, 363-368.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Pseudomonas putida |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 30 | - |
D-Lys | - |
Pseudomonas putida |  |
| 33 | - |
L-Leu | - |
Pseudomonas putida | |
| 36 | - |
L-2-aminobutyrate | - |
Pseudomonas putida | |
| 38 | - |
L-Met | - |
Pseudomonas putida | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 110000 | - |
sedimentation equilibrium method | Pseudomonas putida |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 708.4 | - |
L-Met as substrate | Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Arg | - |
Pseudomonas putida | L-Arg | - |
? | |
| L-2-Aminobutyrate | - |
Pseudomonas putida | D-2-Aminobutyrate | - |
? | |
| L-Ala | - |
Pseudomonas putida | D-Ala | - |
? | |
| L-Ethionine | - |
Pseudomonas putida | D-Ethionine | - |
? | |
| L-Leu | - |
Pseudomonas putida | D-Leu | - |
? | |
| L-Lys | - |
Pseudomonas putida | D-Lys | - |
? | |
| L-Met | - |
Pseudomonas putida | D-Met | - |
? | |
| L-Norleucine | - |
Pseudomonas putida | D-Norleucine | - |
? | |
| L-Norvaline | - |
Pseudomonas putida | D-Norvaline | - |
? | |
| additional information | the relative activity is 1 for Leu, 1.8 for L-2-aminobutyrate, 3.3 for L-Ala, 5.2 for norvaline, 5.9 for L-norleucine, 7.5 for D-Arg, 10.6 for L-Met, 13.2 for D-Lys, and 15.9 for L-ethionine | Pseudomonas putida | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 10 | - |
Pseudomonas putida |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | required for maximal activity | Pseudomonas putida | |
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Release 2023.1 (January 2023)
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