Cloned (Comment) | Organism |
---|---|
gene alr-2, sequence comparisons, the gene encodes a functional enzyme that can complement the alanine racemase deficiency of Escherichia coli strain MB2795, recombinant expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) | Aeromonas hydrophila subsp. hydrophila |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | slightly inhibiting | Aeromonas hydrophila subsp. hydrophila | |
Co2+ | strongly inhibiting | Aeromonas hydrophila subsp. hydrophila | |
DTT | DTT at 1 mmol/l inhibits 67% of the enzyme activity compared with the control and DTT at 5 mmol/l results in complete inhibition of the enzyme activity | Aeromonas hydrophila subsp. hydrophila | |
hydroxylamine | via elimanting the cofactor pyridoxal-5'-phosphate from the enzyme | Aeromonas hydrophila subsp. hydrophila | |
Mg2+ | slightly inhibiting | Aeromonas hydrophila subsp. hydrophila | |
additional information | the enzyme activity is not sensitive to the metal chelating agent EDTA indicating that divalent cations are not required for enzyme activity | Aeromonas hydrophila subsp. hydrophila | |
Na+ | slightly inhibiting | Aeromonas hydrophila subsp. hydrophila | |
Zn2+ | strongly inhibiting | Aeromonas hydrophila subsp. hydrophila |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
15.36 | - |
D-alanine | recombinant His-tagged wild-type enzyme, pH and temperature not specified in the publication | Aeromonas hydrophila subsp. hydrophila | |
35.9 | - |
L-alanine | recombinant His-tagged wild-type enzyme, pH and temperature not specified in the publication | Aeromonas hydrophila subsp. hydrophila |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | enhances the catalytic activity up to 300% | Aeromonas hydrophila subsp. hydrophila | |
additional information | the enzyme activity is not sensitive to the metal chelating agent EDTA indicating that divalent cations are not required for enzyme activity | Aeromonas hydrophila subsp. hydrophila |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanine | Aeromonas hydrophila subsp. hydrophila | - |
D-alanine | - |
r | |
L-alanine | Aeromonas hydrophila subsp. hydrophila HBNUAh01 | - |
D-alanine | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aeromonas hydrophila subsp. hydrophila | A0KL98 | strain ATCC 7966 / DSM 30187 / JCM 1027 / KCTC 2358 / NCIMB 9240, very similar to strain HBNUAh01 | - |
Aeromonas hydrophila subsp. hydrophila HBNUAh01 | A0KL98 | strain ATCC 7966 / DSM 30187 / JCM 1027 / KCTC 2358 / NCIMB 9240, very similar to strain HBNUAh01 | - |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Aeromonas hydrophila subsp. hydrophila |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanine | - |
Aeromonas hydrophila subsp. hydrophila | D-alanine | - |
r | |
L-alanine | - |
Aeromonas hydrophila subsp. hydrophila HBNUAh01 | D-alanine | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 40390, sequence calculation, x * 43000, recombinant His-tagged enzyme, SDS-PAGE | Aeromonas hydrophila subsp. hydrophila |
Synonyms | Comment | Organism |
---|---|---|
alr-2 | - |
Aeromonas hydrophila subsp. hydrophila |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
50 | purified recombinant enzyme, stable up to, over 90% activity remaining | Aeromonas hydrophila subsp. hydrophila |
60 | - |
purified recombinant enzyme, inactivation | Aeromonas hydrophila subsp. hydrophila |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | 11 | purified recombinant enzyme, stable at, over 80% activity remaining | Aeromonas hydrophila subsp. hydrophila |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | PLP, dependent on, no catalytic enzyme activity without, the apoenzyme activity is completely inhibited | Aeromonas hydrophila subsp. hydrophila |