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Literature summary for 5.1.1.1 extracted from
- Characterization of endogenous pyridoxal 5-phosphate-dependent alanine racemase from Bacillus pseudofirmus OF4 (2009), J. Biosci. Bioeng., 107, 225-229.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| subcloned in a D-alanine auxotrophic Escherichia coli strain MB2795 and in Escherichia coli BL21(DE3) | Alkalihalophilus pseudofirmus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 14.91 | - |
D-alanine | - |
Alkalihalophilus pseudofirmus |  |
| 41.79 | - |
L-alanine | - |
Alkalihalophilus pseudofirmus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| exosporium | - |
Alkalihalophilus pseudofirmus | 43592 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41000 | - |
SDS-PAGE | Alkalihalophilus pseudofirmus |
| 87000 | - |
gel filtration | Alkalihalophilus pseudofirmus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Alkalihalophilus pseudofirmus | B3VI72 | - |
- |
| Alkalihalophilus pseudofirmus OF4 | B3VI72 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Alkalihalophilus pseudofirmus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| spore | - |
Alkalihalophilus pseudofirmus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3708 | - |
substrate is D-alanine | Alkalihalophilus pseudofirmus |
| 10500 | - |
substrate is L-alanine | Alkalihalophilus pseudofirmus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-alanine | - |
Alkalihalophilus pseudofirmus | D-alanine | - |
r | |
| L-alanine | - |
Alkalihalophilus pseudofirmus OF4 | D-alanine | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 41000, gel filtration and dynamic light scattering | Alkalihalophilus pseudofirmus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alanine racemase | - |
Alkalihalophilus pseudofirmus |
| dadXOF4 | - |
Alkalihalophilus pseudofirmus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Alkalihalophilus pseudofirmus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
70 | between 20 to 50°C the enzyme activity is 75-100%, at 0°C activity is about 28% and at 70°C activity is 20% | Alkalihalophilus pseudofirmus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 10.5 | - |
- |
Alkalihalophilus pseudofirmus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 12.5 | at pH 7.5 and at pH 12.5 activity is just under 40%, at pH 7 and at pH 13 the enzyme is inactive | Alkalihalophilus pseudofirmus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | PLP is bound to the enzyme, adding PLP during developement of enzyme or to an assay is not necessary | Alkalihalophilus pseudofirmus | |
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