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Literature summary for 5.1.1.1 extracted from
- Cloning of alanine racemase genes from Pseudomonas fluorescens strains and oligomerization states of gene products expressed in Escherichia coli (2005), J. Biosci. Bioeng., 100, 409-417.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| strain has one single enzyme gene, expression in Escherichia coli with His-tag | Pseudomonas fluorescens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.7 | - |
D-alanine | pH 7.4, 30°C | Pseudomonas fluorescens |  |
| 8.1 | - |
L-alanine | pH 7.4, 30°C | Pseudomonas fluorescens | |
| 8.7 | - |
D-alanine | pH 7.4, 30°C | Pseudomonas fluorescens | |
| 18.4 | - |
L-alanine | pH 7.4, 30°C | Pseudomonas fluorescens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 39000 | - |
1 * 39000, SDS-PAGE, 1 * 39700, calculated, enzyme shows monomer-dimer equilibrium | Pseudomonas fluorescens |
| 39000 | - |
1 * 39000, SDS-PAGE, 1 * 39800, calculated, enzyme shows monomer-dimer equilibrium | Pseudomonas fluorescens |
| 39000 | - |
2 * 39000, SDS-PAGE, enzyme shows monomer-dimer equilibrium | Pseudomonas fluorescens |
| 39700 | - |
1 * 39000, SDS-PAGE, 1 * 39700, calculated, enzyme shows monomer-dimer equilibrium | Pseudomonas fluorescens |
| 39800 | - |
1 * 39000, SDS-PAGE, 1 * 39800, calculated, enzyme shows monomer-dimer equilibrium | Pseudomonas fluorescens |
| 40000 | - |
gel filtration | Pseudomonas fluorescens |
| 80000 | - |
gel filtration | Pseudomonas fluorescens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas fluorescens | - |
- |
- |
| Pseudomonas fluorescens | - |
soil-borne strain LRB3W1 | - |
| Pseudomonas fluorescens TM5-2 | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-alanine | - |
Pseudomonas fluorescens | L-alanine | - |
r | |
| D-alanine | - |
Pseudomonas fluorescens TM5-2 | L-alanine | - |
r | |
| L-alanine | - |
Pseudomonas fluorescens | D-alanine | - |
r | |
| L-alanine | - |
Pseudomonas fluorescens TM5-2 | D-alanine | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 39000, SDS-PAGE, enzyme shows monomer-dimer equilibrium | Pseudomonas fluorescens |
| monomer | 1 * 39000, SDS-PAGE, 1 * 39700, calculated, enzyme shows monomer-dimer equilibrium | Pseudomonas fluorescens |
| monomer | 1 * 39000, SDS-PAGE, 1 * 39800, calculated, enzyme shows monomer-dimer equilibrium | Pseudomonas fluorescens |
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