Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
- |
Streptomyces lavendulae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-cycloserine | time-dependent inactivation rate of enzyme from Streptomyces lavendulae is slower than for enzyme from Escherichia coli | Escherichia coli | |
D-cycloserine | time-dependent inactivation rate of enzyme from Streptomyces lavendulae is slower than for enzyme from Escherichia coli. Enzyme from Streptomyces lavendulae is one of its self-resistance determinants | Streptomyces lavendulae | |
L-Cycloserine | - |
Escherichia coli | |
additional information | not inhibitory: L-cycloserine | Streptomyces lavendulae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
L-alanine | pH 8.2, 25°C | Streptomyces lavendulae | |
0.7 | - |
D-alanine | pH 8.2, 25°C | Streptomyces lavendulae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Streptomyces lavendulae | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39900 | - |
2 * 39900, calculated, 2 * 42000, SDs-PAGE | Escherichia coli |
39900 | - |
2 * 39900, calculated, 2 * 42000, SDs-PAGE | Streptomyces lavendulae |
42000 | - |
2 * 39900, calculated, 2 * 42000, SDs-PAGE | Escherichia coli |
42000 | - |
2 * 39900, calculated, 2 * 42000, SDs-PAGE | Streptomyces lavendulae |
80000 | - |
gel filtration | Escherichia coli |
80000 | - |
gel filtration | Streptomyces lavendulae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Streptomyces lavendulae | Q65YW7 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Escherichia coli |
recombinant protein | Streptomyces lavendulae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-alanine | - |
Escherichia coli | L-alanine | - |
r | |
D-alanine | - |
Streptomyces lavendulae | L-alanine | - |
r | |
L-alanine | - |
Escherichia coli | D-alanine | - |
r | |
L-alanine | - |
Streptomyces lavendulae | D-alanine | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 39900, calculated, 2 * 42000, SDs-PAGE | Escherichia coli |
dimer | 2 * 39900, calculated, 2 * 42000, SDs-PAGE | Streptomyces lavendulae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
55 | - |
L-alanine | pH 8.2, 25°C | Streptomyces lavendulae | |
70 | - |
D-alanine | pH 8.2, 25°C | Streptomyces lavendulae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.87 | - |
D-cycloserine | pH 8.2, 25°C | Streptomyces lavendulae | |
1.2 | - |
D-cycloserine | pH 8.2, 25°C | Escherichia coli | |
8.2 | - |
L-Cycloserine | pH 8.2, 25°C | Escherichia coli |