Activating Compound | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | 0.02 mM, activation to 108% of control in the direction of D-Ala formation, activation to 124% of control in the direction of L-Ala formation | Penaeus monodon |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Aminooxyacetate | 1 mM, complete inhibition, both directions | Penaeus monodon | |
hydroxylamine | 1 mM, complete inhibition, both directions | Penaeus monodon |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
167 | - |
L-Ala | pH 8.5, 37°C | Penaeus monodon | |
179 | - |
D-Ala | pH 8.5, 37°C | Penaeus monodon |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
2 * 44000, SDS-PAGE | Penaeus monodon |
90000 | - |
gel filtration | Penaeus monodon |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Penaeus monodon | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Penaeus monodon |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
muscle | - |
Penaeus monodon | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2807 | - |
- |
Penaeus monodon |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Ala | - |
Penaeus monodon | D-Ala | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 44000, SDS-PAGE | Penaeus monodon |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
formation of L-Ala or D-Ala | Penaeus monodon |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 50 | 25°C: about 65% of maximal activity, formation of L-Ala or D-Ala, 50°C: about 50% of maximal activity in formation of D-Ala, about 60% of maximal activity in formation of L-Ala | Penaeus monodon |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2314 | - |
D-Ala | pH 8.5, 37°C | Penaeus monodon | |
2589 | - |
L-Ala | pH 8.5, 37°C | Penaeus monodon |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
formation of D-Ala | Penaeus monodon |
10 | - |
formation of L-Ala | Penaeus monodon |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 11 | pH 7.0: about 40% of maximal activity in formation of D-Ala, about 50% of maximal activity in formation of L-Ala, pH 11: about 95% of maximal activity in formation of L-Ala and D-Ala | Penaeus monodon |