Protein Variants | Comment | Organism |
---|---|---|
K39A | mutant enzyme is inactive as a catalyst for racemization as well as transamination | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Ala | the enzyme catalyzes transamination as a side function.The pyridoxal form of the enzyme is converted to the pyridoxamine form by incubation with its natural substrate, D-alanine or L-alanine, under acidic conditions: the enzyme loses its racemase activity concomitantly. The pyridoxamine form of the enzyme returns to the pyridoxal form by incubation with pyruvate at alkaline pH | Geobacillus stearothermophilus | D-Ala | - |
r | |
additional information | the epsilon-amino group of Lys39 participates in both racemization and transamination when catalyzed by the wild-type enzyme | Geobacillus stearothermophilus | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
racemization from D-Ala to L-Ala | Geobacillus stearothermophilus |
10 | - |
racemization from L-Ala to D-Ala | Geobacillus stearothermophilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8.5 | 11 | pH 8.5: about 55% of maximal activity, pH 11.0: about 65% of maximal activity, racemization from L-Ala to D-Ala | Geobacillus stearothermophilus |
9 | 11 | pH 9: about 50% of maximal activity, pH 11.0: about 35% of maximal activity, racemization from D-Ala to L-Ala | Geobacillus stearothermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | the pyridoxal form of the enzyme is converted to the pyridoxamine form by incubation with its natural substrate, D-alanine or L-alanine, under acidic conditions: the enzyme loses its racemase activity concomitantly. The pyridoxamine form of the enzyme returns to the pyridoxal form by incubation with pyruvate at alkaline pH | Geobacillus stearothermophilus |