Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli. Heterologous expression renders Saccharomyces cerevisiae capable of utilization of D-Ala as a nitrogen source but also relieves the yeast from the toxicity of D-Ala | Schizosaccharomyces pombe |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Sodium borohydride | complete inactivation after dialysis against | Schizosaccharomyces pombe |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.4 | - |
D-Ala | 37°C, pH 9.0 | Schizosaccharomyces pombe | |
5 | - |
L-Ala | 37°C, pH 9.0 | Schizosaccharomyces pombe |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
gel filtration | Schizosaccharomyces pombe |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Schizosaccharomyces pombe | O59828 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Schizosaccharomyces pombe |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-2-Aminobutyrate | 0.37% of the activity with L-Ala | Schizosaccharomyces pombe | D-2-Aminobutyrate | - |
r | |
L-Ala | - |
Schizosaccharomyces pombe | D-Ala | - |
r | |
L-Ser | 3.7% of the activity with L-Ala | Schizosaccharomyces pombe | D-Ser | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 44000, SDS-PAGE | Schizosaccharomyces pombe |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
D-Ala as substrate | Schizosaccharomyces pombe |
9.5 | - |
L-Ala as substrate | Schizosaccharomyces pombe |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | coenzyme | Schizosaccharomyces pombe |