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Literature summary for 5.1.1.1 extracted from
- Partial purification and properties of alanine racemase from the muscle of black tiger prawn Penaeus monodon (1997), Fish. Sci., 63, 440-445.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| FAD | slight activation at low concentrations, inhibition at high concentrations | Penaeus monodon |  |
| NaCl | slight inhibition above 600 mM | Penaeus monodon | |
| pyridoxal 5'-phosphate | slight activation at low concentrations, inhibition at high concentrations | Penaeus monodon | |
| pyruvate | - |
Penaeus monodon | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 119 | - |
D-Ala | - |
Penaeus monodon | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 85000 | - |
HPLC gel filtration | Penaeus monodon |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Penaeus monodon | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Penaeus monodon |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| muscle | - |
Penaeus monodon | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 567 | - |
- |
Penaeus monodon |
Storage Stability
| Storage Stability | Organism |
|---|---|
| enzyme loses about 20% of activity during storage for 20 days at -20°C, 0°C and 5°C | Penaeus monodon |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-Ala | - |
Penaeus monodon | D-Ala | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 40 | - |
Penaeus monodon |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 50 | 20°C: about 60% of maximal activity, 50°C, about 35% of maximal activity | Penaeus monodon |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 50 | pH 8.0, 5 min, sharp decrease of activity between 40°C and 50°C | Penaeus monodon |
| 60 | - |
pH 8.0, 5 min, almost complete inactivation | Penaeus monodon |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Penaeus monodon |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9.5 | 7.0: about 45% of maximal activity, 9.5: about 95% of maximal activity | Penaeus monodon |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | not required as cofactor, slight activation at low concentrations, inhibition at high concentrations | Penaeus monodon | |
| pyridoxal 5'-phosphate | not required as cofactor, slight activation at low concentrations, inhibition at high concentrations | Penaeus monodon | |
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