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Literature summary for 5.1.1.1 extracted from

  • Soda, K.; Esaki, N.
    Pyridoxal enzymes acting on D-amino acids (1994), Pure Appl. Chem., 66, 709-714.
No PubMed abstract available
Search for more literature for 5.1.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Geobacillus stearothermophilus

Protein Variants

Protein Variants Comment Organism
additional information mutant gene which tandemly encodes the two polypeptides of the enzyme subunit, fragment 1 and fragment 2, cleaved at the position corresponding to the predicted hinge region. The mutant fragmentary alanine racemase is active at about 40% of the activity of the wild type enzyme Geobacillus stearothermophilus

General Stability

General Stability Organism
in 0.6 M to 1.5 M guanidine hydrochloride the dimeric enzyme is dissociated into a monomeric form, which is catalytically inactive Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-Ala Geobacillus stearothermophilus enzyme provides D-Ala as an essential building block for biosynthesis of the peptidoglycan layer of the cell wall ?
-
 ?
L-Ala Bacillus sp. (in: Bacteria) enzyme provides D-Ala as an essential building block for biosynthesis of the peptidoglycan layer of the cell wall ?
-
 ?
L-Ala Bacillus sp. (in: Bacteria) YM-1 enzyme provides D-Ala as an essential building block for biosynthesis of the peptidoglycan layer of the cell wall ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
-
-
Bacillus sp. (in: Bacteria) YM-1
-
-
-
Geobacillus stearothermophilus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Ala
-
 Geobacillus stearothermophilus D-Ala
-
 ?
L-Ala
-
 Bacillus sp. (in: Bacteria) D-Ala
-
 ?
L-Ala
-
 Bacillus sp. (in: Bacteria) YM-1 D-Ala
-
 ?
L-Ala enzyme provides D-Ala as an essential building block for biosynthesis of the peptidoglycan layer of the cell wall Geobacillus stearothermophilus ?
-
 ?
L-Ala enzyme provides D-Ala as an essential building block for biosynthesis of the peptidoglycan layer of the cell wall Bacillus sp. (in: Bacteria) ?
-
 ?
L-Ala enzyme provides D-Ala as an essential building block for biosynthesis of the peptidoglycan layer of the cell wall Bacillus sp. (in: Bacteria) YM-1 ?
-
 ?