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Literature summary for 5.1.1.1 extracted from
- Thermolabile alanine racemase from a psychrotroph, Pseudomonas fluorescens: purification and properties (1993), Biosci. Biotechnol. Biochem., 57, 93-97.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 12.8 | - |
D-Ala | - |
Pseudomonas fluorescens |  |
| 18.9 | - |
L-Ala | - |
Pseudomonas fluorescens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
2 * 38000, SDS-PAGE | Pseudomonas fluorescens |
| 76000 | - |
gel filtration in presence or absence of 2-mercaptoethanol | Pseudomonas fluorescens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas fluorescens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas fluorescens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 587 | - |
- |
Pseudomonas fluorescens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for 1 month | Pseudomonas fluorescens |
| 4°C, stable for 1 week | Pseudomonas fluorescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-Ala | - |
Pseudomonas fluorescens | D-Ala | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 38000, SDS-PAGE | Pseudomonas fluorescens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Pseudomonas fluorescens |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
40 | 0°C: about 25% of maximal activity, 40°C: about 45% of maximal activity | Pseudomonas fluorescens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
1 h, stable | Pseudomonas fluorescens |
| 30 | - |
over 30°C, 1 h, quick loss of activity | Pseudomonas fluorescens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.3 | - |
- |
Pseudomonas fluorescens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | 2 mol of pyridoxal 5'-phosphate bound per mol of enzyme dimer | Pseudomonas fluorescens | |
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