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Literature summary for 5.1.1.1 extracted from

  • Inagaki, K.; Tanizawa, K.; Badet, B.; Walsh, C.T.; Tanaka, H.; Soda, K.
    Thermostable alanine racemase from Bacillus stearothermophilus: molecular cloning of the gene, enzyme purification, and characterization (1986), Biochemistry, 25, 3268-3274.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli C600 Geobacillus stearothermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7
-
L-Ala D-Ala Geobacillus stearothermophilus
4.25
-
L-Ala
-
Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
2 * 39000, SDS-PAGE Geobacillus stearothermophilus
78000
-
equilibrium sedimentation method Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Ala specific for Ala Geobacillus stearothermophilus D-Ala
-
?

Subunits

Subunits Comment Organism
dimer 2 * 39000, SDS-PAGE Geobacillus stearothermophilus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate 2 mol of pyridoxal 5'-phosphate bound per mol of enzyme dimer Geobacillus stearothermophilus