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Literature summary for 4.99.1.9 extracted from

  • Hansson, M.; Hederstedt, L.
    Purification and characterisation of a water-soluble ferrochelatase from Bacillus subtilis (1994), Eur. J. Biochem., 220, 201-208.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene cloned in pUC18, expression in Escherichia coli JM109/pLUGlSe2 Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
N-ethylmaleimide 2 mM, 99% inactivation after incubation for 2 h. Protoporphyrin IX or Zn2+ at concentrations of 0.8 mM and 20 mM, respectively, does not protect Bacillus subtilis
p-chloromercuriphenylsulfonate
-
Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.008
-
protoporphyrin IX pH 7.2, 30°C. The Km-value for protoporphyrin IX is the same independent of whether Zn2+ or Fe2+ is used in the reaction Bacillus subtilis
0.017
-
Zn2+ pH 7.2, 30°C Bacillus subtilis
0.17
-
Cu2+ pH 7.2, 30°C Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35348
-
1 * 35348, calculated from sequence Bacillus subtilis
40000
-
gel filtration Bacillus subtilis
40500
-
1 * 40500, SDS-PAGE Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bacillus subtilis

Storage Stability

Storage Stability Organism
-20°C, the purified enzyme is stable for at least five months Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
protoporphyrin IX + Cu2+
-
Bacillus subtilis Cu-protoporphyrin IX + 2 H+
-
?
protoporphyrin IX + Fe2+ the enzyme shows no detectable activity with Ca2+, Co2+, Fe3+, Mg2+, or Rb+ Bacillus subtilis Fe-protoporphyrin IX + 2 H+
-
?
protoporphyrin IX + Zn2+
-
Bacillus subtilis Zn-protoporphyrin IX + 2 H+
-
?

Subunits

Subunits Comment Organism
monomer 1 * 40500, SDS-PAGE Bacillus subtilis
monomer 1 * 35348, calculated from sequence Bacillus subtilis

Synonyms

Synonyms Comment Organism
hemH
-
Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Bacillus subtilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.4
-
protoporphyrin IX pH 7.2, 30°C. The Km-value for protoporphyrin IX is the same independent of whether Zn2+ or Fe2+ is used in the reaction Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
assay at Bacillus subtilis

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8 pH 6.5: about 50% of maximal activity, pH 8.0: about 50% of maximal activity Bacillus subtilis

General Information

General Information Comment Organism
metabolism the enzyme participates in heme biosynthesis Bacillus subtilis