Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
C135A | the mutant shows increased specific activity compared to the wild type enzyme | Arabidopsis thaliana |
C199A | the mutant shows reduced specific activity compared to the wild type enzyme | Arabidopsis thaliana |
C210A | the mutant shows increased specific activity compared to the wild type enzyme | Arabidopsis thaliana |
C213A | the mutant shows reduced specific activity compared to the wild type enzyme | Arabidopsis thaliana |
C219A | the mutant shows increased specific activity compared to the wild type enzyme | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Arabidopsis thaliana | 9507 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | contains 1.93 mol Fe2+ per mol of protein | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18600 | - |
2 * 18600, estimated from SDS-PAGE | Arabidopsis thaliana |
36010 | - |
calculated from gel filtration | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sirohydrochlorin + Fe2+ | Arabidopsis thaliana | - |
siroheme + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Arabidopsis thaliana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
33.25 | - |
mutant enzyme C199A, in 20 mM Tris/HCl (pH 8.0) and 10 mM NaCl, at 23°C | Arabidopsis thaliana |
36.08 | - |
mutant enzyme C213A, in 20 mM Tris/HCl (pH 8.0) and 10 mM NaCl, at 23°C | Arabidopsis thaliana |
38.5 | - |
wild type enzyme, in 20 mM Tris/HCl (pH 8.0) and 10 mM NaCl, at 23°C | Arabidopsis thaliana |
43 | - |
mutant enzyme C219A, in 20 mM Tris/HCl (pH 8.0) and 10 mM NaCl, at 23°C | Arabidopsis thaliana |
44.67 | - |
mutant enzyme C135A, in 20 mM Tris/HCl (pH 8.0) and 10 mM NaCl, at 23°C | Arabidopsis thaliana |
55.17 | - |
mutant enzyme C210A, in 20 mM Tris/HCl (pH 8.0) and 10 mM NaCl, at 23°C | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sirohydrochlorin + Fe2+ | - |
Arabidopsis thaliana | siroheme + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 18600, estimated from SDS-PAGE | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
SirB | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
4Fe-4S-center | - |
Arabidopsis thaliana |