Crystallization (Comment) | Organism |
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X-ray crystal structure of Met8p | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
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D141A | mutant of bifunctional Met8p is devoid of both dehydrogenase and ferrochelatase activities | Saccharomyces cerevisiae |
G22D | mutant of bifunctional Met8p is completely inactive as NAD+-dependent dehydrogenase, but functions as ferrochelatase | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
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sirohydrochlorin + Fe2+ | Saccharomyces cerevisiae | Met8p catalyzes ferrochelation during the biosynthesis of siroheme | siroheme + 2 H+ | - |
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Organism | UniProt | Comment | Textmining |
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Saccharomyces cerevisiae | - |
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Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
siroheme + 2 H+ = sirohydrochlorin + Fe2+ | the enzyme from Pseudomonas chloroaphis contains Ca2+ and protoheme IX, the iron of which must be in the form Fe2+ for activity, the enzyme exhibits a strong preference for aliphatic aldoximes, such as butyraldoxime and acetaldoxime, over aromatic aldoximes, such as pyridine-2-aldoxime, which is a poor substrate, no activity was found with the aromatic aldoximes benzaldoxime and pyridine-4-aldoxime, mechanism | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sirohydrochlorin + Fe2+ | Met8p catalyzes ferrochelation during the synthesis of siroheme, both ferrochelation and NAD+-dependent dehydrogenation of preccorin-2 to produce sirohydrochlorin take place in a single bifunctional active site, Asp-141 participates in both catalytic reactions, which are not linked mechanistically, mechanism | Saccharomyces cerevisiae | siroheme + 2 H+ | - |
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sirohydrochlorin + Fe2+ | Met8p catalyzes ferrochelation during the biosynthesis of siroheme | Saccharomyces cerevisiae | siroheme + 2 H+ | - |
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Subunits | Comment | Organism |
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homodimer | each monomer is composed of three functional domains, domain structure | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
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Met8p | Saccharomyces cerevisiae | Saccharomyces cerevisiae |
More | member of the class 2 chelatases | Saccharomyces cerevisiae |