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Literature summary for 4.99.1.3 extracted from

  • Bali, S.; Rollauer, S.; Roversi, P.; Raux-Deery, E.; Lea, S.M.; Warren, M.J.; Ferguson, S.J.
    Identification and characterization of the missing terminal enzyme for siroheme biosynthesis in alpha-proteobacteria (2014), Mol. Microbiol., 92, 153-163.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 codonplus (RIPL) cells Paracoccus pantotrophus

Protein Variants

Protein Variants Comment Organism
H127A inactive Paracoccus pantotrophus
H187A inactive Paracoccus pantotrophus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
x * 23000, SDS-PAGE Paracoccus pantotrophus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cobalt-sirohydrochlorin + H+ Paracoccus denitrificans
-
sirohydrochlorin + Co2+
-
?
cobalt-sirohydrochlorin + H+ Paracoccus pantotrophus
-
sirohydrochlorin + Co2+
-
?

Organism

Organism UniProt Comment Textmining
Paracoccus denitrificans
-
-
-
Paracoccus pantotrophus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-Sepharose column chromatography Paracoccus pantotrophus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cobalt-sirohydrochlorin + H+
-
Paracoccus denitrificans sirohydrochlorin + Co2+
-
?
cobalt-sirohydrochlorin + H+
-
Paracoccus pantotrophus sirohydrochlorin + Co2+
-
?

Subunits

Subunits Comment Organism
? x * 23000, SDS-PAGE Paracoccus pantotrophus

Synonyms

Synonyms Comment Organism
CbiX
-
Paracoccus denitrificans
CbiX
-
Paracoccus pantotrophus
cobaltochelatase
-
Paracoccus denitrificans
cobaltochelatase
-
Paracoccus pantotrophus
sirohydrochlorin-ferrochelatase
-
Paracoccus denitrificans
sirohydrochlorin-ferrochelatase
-
Paracoccus pantotrophus

General Information

General Information Comment Organism
malfunction a cbiX-deficient mutant is unable to respire anaerobically on nitrate Paracoccus denitrificans