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Literature summary for 4.6.1.2 extracted from

  • Seeger, F.; Quintyn, R.; Tanimoto, A.; Williams, G.J.; Tainer, J.A.; Wysocki, V.H.; Garcin, E.D.
    Interfacial residues promote an optimal alignment of the catalytic center in human soluble guanylate cyclase: heterodimerization is required but not sufficient for activity (2014), Biochemistry, 53, 2153-2165.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.6.1.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 20% (w/v) PEG3350, 50 mM HEPES (pH 7.0), and 1% (w/v) tryptone Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Homo sapiens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activates Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP Homo sapiens
-
 3',5'-cyclic GMP + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q02108
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel HisTrap affinity column chromatography, HiTrap column chromatography, and Superdex 200 or Superdex 75 gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP
-
 Homo sapiens 3',5'-cyclic GMP + diphosphate
-
 ?

Subunits

Subunits Comment Organism
heterodimer
-
 Homo sapiens

Synonyms

Synonyms Comment Organism
sGC
-
 Homo sapiens
soluble guanylate cyclase
-
 Homo sapiens