Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.6.1.2 extracted from

  • James, L.R.; Griffiths, C.H.; Garthwaite, J.; Bellamy, T.C.
    Inhibition of nitric oxide-activated guanylyl cyclase by calmodulin antagonists (2009), Br. J. Pharmacol., 158, 1454-1464.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
nitric oxide
-
Bos taurus
protoporphyrin IX 0.001 mM activates untreated sGC with around half the effectiveness of nitric oxide Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ ionophore A23187 Ca2+-dependent inhibition of sGC can be achieved with the Ca2+ ionophore A23187 (0.01 mM) Bos taurus
Calmidazolium uncompetitive inhibition, time-dependent inhibition at 0.03 mM, pre-treatment of sGC with calmidazolium, followed by filtration, causes an 80% inhibition of sGC activation by nitric oxide and a 23% inhibition of activation by protoporphyrin IX Bos taurus
N-benzyl-N-(2-chloroethyl)-1-phenoxypropan-2-amine
-
Bos taurus
Trifluoperazine
-
Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP Bos taurus
-
3',5'-cyclic-GMP + diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
lung
-
Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP
-
Bos taurus 3',5'-cyclic-GMP + diphosphate
-
?

Synonyms

Synonyms Comment Organism
alpha1beta1 guanylyl cyclase
-
Bos taurus
sGC
-
Bos taurus
soluble guanylyl cyclase
-
Bos taurus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.01
-
purified sGC Bos taurus Calmidazolium

Expression

Organism Comment Expression
Bos taurus pre-treatment of sGC with Tween-20 (0.5%), followed by filtration to remove the detergent, decreases the effectiveness of nitric oxide for activating cGMP synthesis by 73% down