Activating Compound | Comment | Organism | Structure |
---|---|---|---|
3-ethyl-3-(ethylaminoethyl)-1-hydroxy-2-oxo-1-triazene | i.e. NOC-12 | Homo sapiens | |
NO | 340fold activation | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression of His-tagged soluble guanylate cyclase in Spodoptera frugiperda SF9 cells using the baculovirus transfection method | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0498 | - |
GTP | pH 7.5, 37°C, NO-stimulated recombinant enzyme | Homo sapiens | |
0.0901 | - |
GTP | pH 7.5, 37°C, not stimulated recombinant enzyme | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Homo sapiens | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
1 * 77000, alpha-subunit, + 1 * 70000, beta-subunit, SDS-PAGE | Homo sapiens |
77000 | - |
1 * 77000, alpha-subunit, + 1 * 70000, beta-subunit, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP | Homo sapiens | - |
3',5'-cyclic GMP + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant soluble guanylate cyclase from Spodoptera frugiperda SF9 cells by anion exchange chromatography, nickel affinity chromatography, and again anion exchange chromatography on a porus resin | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP | - |
Homo sapiens | 3',5'-cyclic GMP + diphosphate | - |
? | |
additional information | structure-function relationship, overview | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 77000, alpha-subunit, + 1 * 70000, beta-subunit, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
sGC | - |
Homo sapiens |
soluble guanylate cyclase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
GTP | pH 7.5, 37°C, not stimulated recombinant enzyme | Homo sapiens | |
28.7 | - |
GTP | pH 7.5, 37°C, NO-stimulated recombinant enzyme | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | one heme per heterodimer | Homo sapiens |