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Literature summary for 4.6.1.2 extracted from

  • Mathis, K.J.; Emmons, T.L.; Curran, D.F.; Day, J.E.; Tomasselli, A.G.
    High yield purification of soluble guanylate cyclase from bovine lung (2008), Protein Expr. Purif., 60, 58-63.
    View publication on PubMed
Search for more literature for 4.6.1.2

Activating Compound

Activating Compound Comment Organism Structure
3-ethyl-3-(ethylaminoethyl)-1-hydroxy-2-oxo-1-triazene stimulates via release of NO Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics, purified recombinant sGC, overview Bos taurus
0.043
-
 GTP purified recombinant sGC in presence of 3-ethyl-3-(ethylaminoethyl)-1-hydroxy-2-oxo-1-triazene Bos taurus
0.18
-
 GTP purified recombinant sGC Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Bos taurus 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70500
-
 1 * 77532, alpha subunit, + 1 * 70500, beta subunit, SDS-PAGE Bos taurus
77532
-
 1 * 77532, alpha subunit, + 1 * 70500, beta subunit, SDS-PAGE Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP Bos taurus
-
 3',5'-cyclic GMP + diphosphate cGMP is involved in transmitting the NO activating signals to a variety of downstream effectors such as cyclicnucleotide-gated channels, protein kinases, and phosphodiesterases ?

Organism

Organism UniProt Comment Textmining
Bos taurus P16068 beta-subunit
-
Bos taurus P19687 alpha-subunit
-

Purification (Commentary)

Purification (Comment) Organism
native soluble guanylate cyclase from lung to high yield by two different steps of anion chromatography, hydroxyapatite chromatography, a third anion exchange chromatography, GTP affinity chromatography, and gel filtration Bos taurus

Reaction

Reaction Comment Organism Reaction ID
GTP = 3',5'-cyclic GMP + diphosphate reaction mechanism, overview Bos taurus
a

Source Tissue

Source Tissue Comment Organism Textmining
lung
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.022
-
 purified recombinant sGC Bos taurus
1.33
-
 purified recombinant sGC in presence of 3-ethyl-3-(ethylaminoethyl)-1-hydroxy-2-oxo-1-triazene Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP
-
 Bos taurus 3',5'-cyclic GMP + diphosphate
-
 ?
GTP
-
 Bos taurus 3',5'-cyclic GMP + diphosphate cGMP is involved in transmitting the NO activating signals to a variety of downstream effectors such as cyclicnucleotide-gated channels, protein kinases, and phosphodiesterases ?

Subunits

Subunits Comment Organism
dimer 1 * 77532, alpha subunit, + 1 * 70500, beta subunit, SDS-PAGE Bos taurus

Synonyms

Synonyms Comment Organism
sGC
-
 Bos taurus
soluble guanylate cyclase
-
 Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Bos taurus

Cofactor

Cofactor Comment Organism Structure
heme one heme per heterodimer Bos taurus