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Literature summary for 4.6.1.2 extracted from

  • Derbyshire, E.R.; Gunn, A.; Ibrahim, M.; Spiro, T.G.; Britt, R.D.; Marletta, M.A.
    Characterization of two different five-coordinate soluble guanylate cyclase ferrous-nitrosyl complexes (2008), Biochemistry, 47, 3892-3899.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
BAY 41-2272 an allosteric activator Rattus norvegicus
NO binding of NO to sGC leads to the formation of a five-coordinate ferrous-nitrosyl complex and a several hundred-fold increase in cGMP synthesis, the NO activation of sGC is influenced by GTP and the allosteric activators YC-1 and BAY 41-2272, analysis of the Fe-NO conformation, overview Rattus norvegicus
YC-1 an allosteric activator Rattus norvegicus

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type alpha1beta2 sGC enzyme in Spodoptera frugiperda Sf9 cells, and of heme domain mutants in Escherichia coli Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
additional information construction of heme domain mutant comprising residues beta1(1-194) or beta2(1-217) Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Rattus norvegicus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ part of the heme cofactor, analysis of the Fe-NO conformation, overview Rattus norvegicus
Mg2+
-
Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP Rattus norvegicus
-
3',5'-cyclic GMP + diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP
-
Rattus norvegicus 3',5'-cyclic GMP + diphosphate
-
?

Synonyms

Synonyms Comment Organism
sGC
-
Rattus norvegicus
soluble guanylate cyclase
-
Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
heme sGC is a hemoprotein Rattus norvegicus