Literature summary for 4.6.1.2 extracted from

Rothkegel, C.; Schmidt, P.M.; Atkins, D.J.; Hoffmann, L.S.; Schmidt, H.H.; Schroeder, H.; Stasch, J.P.
Dimerization region of soluble guanylate cyclase characterized by bimolecular fluorescence complementation in vivo (2007), Mol. Pharmacol., 72, 1181-1190.

Activating Compound

Activating Compound	Comment	Organism
2-(N,N-diethylamino)-diazenolate-2-oxide	stimulates 4.3fold	Rattus norvegicus
4-((4-carboxybutyl){2-[(4-phenethyl-benzyl)oxy]-phenethyl}amino)methyl[benzoic]acid	NO- and heme-independent sGC activator, stimulates 15fold, stimulation increases up to 30fold in the presence of 1H-(1,2,4)-oxadiazole-(4,3-a)-quinoxalin-1-one	Rattus norvegicus
5-cyclopropyl-2-[1-(2-fluoro-benzyl)-1H-pyrazolo[3,4-b]pyridin-3-yl]-pyrimidin-4-ylamine	NO-independent but heme-dependent sGC stimulator, stimulates the enzyme in a concentration-dependent manner	Rattus norvegicus

Application

Application	Comment	Organism
additional information	segments spanning amino acids alpha1 363-372, alpha1 403-422, alpha1 440-459, beta1 212-222, beta1 304-333, beta1 344-363, and beta1 381-400 within the predicted dimerization region are involved in the process of heterodimerization and therefore in expression of functional sGC	Rattus norvegicus

Cloned(Commentary)

Cloned (Comment)	Organism
construction of a fluorescent alpha1/beta1-sGC heterodimer by fusion of the N-terminal YFP fragment and the C-terminal fragment to the N terminus and the C terminus of the alpha1- and beta1-subunit, respectively. Generated fusion proteins, wild-type and deletion mutants transiently expressed in a cGMP reporter cell line based on a Chinese hamster ovary cell line stably transfected with the cyclic nucleotide gated olfactory CNG2A-channel and cytosolic aequorin	Rattus norvegicus

Cloned (Comment)

Organism

construction of a fluorescent alpha1/beta1-sGC heterodimer by fusion of the N-terminal YFP fragment and the C-terminal fragment to the N terminus and the C terminus of the alpha1- and beta1-subunit, respectively. Generated fusion proteins, wild-type and deletion mutants transiently expressed in a cGMP reporter cell line based on a Chinese hamster ovary cell line stably transfected with the cyclic nucleotide gated olfactory CNG2A-channel and cytosolic aequorin

Rattus norvegicus

Protein Variants

Protein Variants	Comment	Organism
additional information	alpha1-sGC and beta1-sGC deletion mutants, coexpression of alpha1-YNV deletion mutants with wild-type-beta1-YCV as well as beta1-YCV deletion mutants with wild-type-beta1-YNV-sGC results in functional heterodimerization of both sGC subunits in the cytosol of the cell	Rattus norvegicus

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	-	-	-

Subunits

Subunits	Comment	Organism
heterodimer	deletion mutants, bimolecular fluorescence complementation	Rattus norvegicus

Synonyms

Synonyms	Comment	Organism
sGC	-	Rattus norvegicus
soluble guanylate cyclase	-	Rattus norvegicus