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Literature summary for 4.6.1.2 extracted from

  • Veltman, D.M.; Van Haastert, P.J.
    Guanylyl cyclase protein and cGMP product independently control front and back of chemotaxing Dictyostelium cells (2006), Mol. Biol. Cell, 17, 3921-3929.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.6.1.2

Application

Application Comment Organism
additional information cGMP suppresses pseudopod formation in the back of the cell, whereas the sGC protein refines pseudopod formation at the leading edge Dictyostelium discoideum

Cloned(Commentary)

Cloned (Comment) Organism
wild-type sGC and mutant sGC constructs fused at the C-terminus to GFP and expressed in gc-null cells Dictyostelium discoideum

Protein Variants

Protein Variants Comment Organism
additional information mutant sGCdeltacat with point mutation introduced in the catalytic site, loses catalytic activity, shows poor myosin localization at the back, but excellent localization of the sGC protein at the leading edge, where it enhances the probability that a new pseudopod is made in proximity to previous pseudopodia, resulting in a decrease of the degree of turning. Mutant sGCdeltaN with deletion of the N-terminal 877 amino acids, exhibits excellent cGMP formation and myosin localization in the back of the cell, but exhibits poor orientation at the leading edge Dictyostelium discoideum

Organism

Organism UniProt Comment Textmining
Dictyostelium discoideum Q963L5
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-

Source Tissue

Source Tissue Comment Organism Textmining
pseudopodium sGC protein enriched at the leading edge of the cell during chemotaxis Dictyostelium discoideum
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP
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 Dictyostelium discoideum 3',5'-cyclic GMP + diphosphate
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 ?

Synonyms

Synonyms Comment Organism
sGC
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 Dictyostelium discoideum
soluble guanylyl cyclase
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 Dictyostelium discoideum