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Literature summary for 4.6.1.2 extracted from
- Native human nitric oxide sensitive guanylyl cyclase: purification and characterization (2004), Biochem. Pharmacol., 67, 1579-1585.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2,2-diethyl-1-nitroso-oxyhydrazine | 0.1 mM, 87fold increase in activity, NO-relasing substance | Homo sapiens |  |
| NO | - |
Homo sapiens | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Sf9 insect cells | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.057 | - |
GTP | in the presence of NO | Homo sapiens | |
| 0.19 | - |
GTP | in the absence of NO | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 72000 | - |
1 * 72000 + 1 * 80000, SDS-PAGE | Homo sapiens |
| 80000 | - |
1 * 72000 + 1 * 80000, SDS-PAGE | Homo sapiens |
| 160000 | - |
gel filtration | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP | Homo sapiens | - |
3',5'-cyclic GMP + diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Q-Sepharose, Blue-Sepharose, hydroxyapatite, gel filtration | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| platelet | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.034 | - |
basal activity in the absence of NO | Homo sapiens |
| 2.96 | - |
in the presence of 0.1 mM 2,2-diethyl-1-nitroso-oxyhydrazine as NO source | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP | - |
Homo sapiens | 3',5'-cyclic GMP + diphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 72000 + 1 * 80000, SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| nitric oxid sensitive guanylyl cyclase | - |
Homo sapiens |
| sGC | - |
Homo sapiens |
| soluble guanylyl cyclase | - |
Homo sapiens |
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