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Literature summary for 4.6.1.2 extracted from

  • Ohlstein, E.H.; Wood, K.S.; Ignarro, L.J.
    Purification and properties of heme-deficient hepatic soluble guanylate cyclase: effects of heme and other factors on enzyme activation by NO, NO-heme, and protoporphyrin IX (1982), Arch. Biochem. Biophys., 218, 187-198.
    View publication on PubMed
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 GTP
-
 Rattus norvegicus
0.085
-
 GTP
-
 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Rattus norvegicus
-
-

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Storage Stability

Storage Stability Organism
-60°C, N2 atmosphere, 25 mM triethanolamine, pH 7.8, 5 mM dithiothreitol, 1 M NaCl, 30% glycerol, several months Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP GTP in form of MgGTP2- Rattus norvegicus 3',5'-cyclic GMP + diphosphate
-
 ?
GTP GTP in form of MnGTP2- Rattus norvegicus 3',5'-cyclic GMP + diphosphate
-
 ?