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Literature summary for 4.6.1.14 extracted from

  • Subramanya, S.; Mensa-Wilmot, K.
    Regulated cleavage of intracellular glycosylphosphatidylinositol in a trypanosome. Peroxisome-to-endoplasmic reticulum translocation of a phospholipase C (2006), FEBS J., 273, 2110-2126.
    View publication on PubMed
Search for more literature for 4.6.1.14

Cloned(Commentary)

Cloned (Comment) Organism
mutants subcloned into pLew82(DLUC) vector, Cys mutants stably expressed in strain RUMP528 Trypanosoma brucei

Protein Variants

Protein Variants Comment Organism
C184A exhibits properties indistinguishable from the unmutated enzyme, in exiting glycosomes after exposure to pH 9.1 or hypotonic buffer Trypanosoma brucei
C24A exhibits properties indistinguishable from the unmutated enzyme, in exiting glycosomes after exposure to pH 9.1 or hypotonic buffer Trypanosoma brucei
C269S/C270S exhibits properties indistinguishable from the unmutated enzyme, in exiting glycosomes after exposure to pH 9.1 or hypotonic buffer Trypanosoma brucei
C269S/C270S/C273S exhibits properties indistinguishable from the unmutated enzyme, in exiting glycosomes after exposure to pH 9.1 or hypotonic buffer Trypanosoma brucei
C269S/C273S exhibits properties indistinguishable from the unmutated enzyme, in exiting glycosomes after exposure to pH 9.1 or hypotonic buffer Trypanosoma brucei
C270S/C273S exhibits properties indistinguishable from the unmutated enzyme, in exiting glycosomes after exposure to pH 9.1 or hypotonic buffer Trypanosoma brucei
C332A exhibits properties indistinguishable from the unmutated enzyme, in exiting glycosomes after exposure to pH 9.1 or hypotonic buffer Trypanosoma brucei
C347A can not exit glycosomes after treatment of cells expressing the protein with mild base or hypo-osmotic buffer Trypanosoma brucei
C80A exhibits properties indistinguishable from the unmutated enzyme, in exiting glycosomes after exposure to pH 9.1 or hypotonic buffer Trypanosoma brucei
additional information strain RUMP528, mutant GPI-PLC-/- cells, only 10% loss of alpha-toxin signal in cells exposed to hypo-osmotic buffer Trypanosoma brucei
Q81L fails to cleave glycosylphosphatidylinositols despite being transported from glycosomes to the endoplasmic reticulum after hypoosmotic or mild alkaline treatment of parasites, lacks enzyme activity Trypanosoma brucei

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum Cys347 is part of a peptide motif required for post-translational targeting of GPI-PLC to the endoplasmic reticulum Trypanosoma brucei 5783
-
peroxisome enzyme is translocated from glycosomes to the endoplasmic reticulum when cells are treated with mild alkaline or hypo-osmotic conditions Trypanosoma brucei 5777
-

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei
-
-
-
Trypanosoma brucei 427
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Trypanosoma brucei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycolipid A is cleaved when Trypanosoma brucei encounters a mildly alkaline or hypotonic extracellular environment Trypanosoma brucei ?
-
 ?
glycolipid A is cleaved when Trypanosoma brucei encounters a mildly alkaline or hypotonic extracellular environment Trypanosoma brucei 427 ?
-
 ?
glycolipid Y
-
 Trypanosoma brucei ?
-
 ?
glycolipid Y
-
 Trypanosoma brucei 427 ?
-
 ?
additional information does not digest glycolipid Z and glycolipid C Trypanosoma brucei ?
-
 ?
additional information does not digest glycolipid Z and glycolipid C Trypanosoma brucei 427 ?
-
 ?
variant surface glycoprotein on exposure to mild alkali Trypanosoma brucei ?
-
 ?
variant surface glycoprotein on exposure to mild alkali Trypanosoma brucei 427 ?
-
 ?

Synonyms

Synonyms Comment Organism
glycosylphosphatidylinositol-specific phospholipase C
-
 Trypanosoma brucei
GPI-PLC
-
 Trypanosoma brucei

pH Range

pH Minimum pH Maximum Comment Organism
7.4 9.1
-
 Trypanosoma brucei